AB182577

Anti-ACTR3B antibody [EPR14091]

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal ACTR3B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

ARP11, ARP4, ACTR3B, Actin-related protein 3B, ARP3-beta, Actin-like protein 3B, Actin-related protein ARP4

2 이미지

Supplier Data

Immunoprecipitation - Anti-ACTR3B antibody [EPR14091] (AB182577)

Immunoprecipitation analysis of Human fetal brain lysate labeling ACTR3B using ab182577 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-ACTR3B antibody [EPR14091] (ab182577)

Predicted band size: 48 kDa

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Supplier Data

Western blot - Anti-ACTR3B antibody [EPR14091] (AB182577)

All lanes:

Western blot - Anti-ACTR3B antibody [EPR14091] (ab182577) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14091

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ACTR3B also known as Actin-related protein 3B is a component of the ARP2/3 complex which contains approximately 47 kDa. Mechanically ACTR3B plays a role in the regulation of actin polymerization which is essential for the formation of branched actin networks. ACTR3B expression occurs in various tissues with prominent levels in cells requiring dynamic cytoskeletal changes. It interacts closely with other proteins of the ARP2/3 complex to drive actin network formation.

Biological function summary

ACTR3B influences cellular processes related to the maintenance of cell shape and motility by being part of the ARP2/3 complex. This complex assists in the nucleation of new actin filaments contributing to the dynamic remodeling of the actin cytoskeleton. The activity of ACTR3B impacts cell migration cell division and intracellular transport by reorganizing actin structures. ACTR3B's role within the ARP2/3 complex underlines its involvement in cellular projects demanding changes to cell morphology and interaction with the environment.

Pathways

The involvement of ACTR3B in the regulation of actin polymerization positions it within the WASP/WAVE signaling pathways. These pathways orchestrate the actin cytoskeletal dynamics that direct important cellular movements such as lamellipodia formation. ACTR3B works alongside proteins like WASP (Wiskott-Aldrich Syndrome Protein) in its pathway activities supporting coordinated cellular responses to external and internal signals through cytoskeletal rearrangement.

ACTR3B's association with actin polymerization links it to cancers and immune system diseases including Wiskott-Aldrich syndrome. These diseases often involve defects in cell movement and signaling due to impaired actin dynamics. In the context of cancer altered expression or mutation of ACTR3B may support metastatic processes through disrupted cell adhesion and migration. Additionally ACTR3B's connection with WASP highlights its potential role in disorders where actin cytoskeletal defects significantly affect immune responses.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in the organization of the actin cytoskeleton. May function as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. May decrease the metastatic potential of tumors.

See full target information ACTR3B

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncoimmunology 12:2173422 PubMed36776524

2023

Gankyrin-mediated interaction between cancer cells and tumor-associated macrophages facilitates prostate cancer progression and androgen deprivation therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Peng,Chao Wang,Hongru Wang,Min Qu,Keqin Dong,Yongwei Yu,Yuquan Jiang,Sishun Gan,Xu Gao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACTR3B antibody [EPR14091]

Immunoprecipitation - Anti-ACTR3B antibody [EPR14091] (AB182577)

Western blot - Anti-ACTR3B antibody [EPR14091] (AB182577)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise