JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317114

Anti-Acylglycerol kinase antibody [HL1846]

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal AGK antibody. Suitable for WB and reacts with Transfected cell lysate, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human AGK.

대체 명칭 보기

MULK, AGK, hAGK, Multiple substrate lipid kinase, HsMuLK, MuLK, Multi-substrate lipid kinase

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1846

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human AGK.

Q53H12

style
left-column

보완 제품

Primary Antibodies

AB131272

Anti-ACTL6A antibody [EPR7443]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL6A antibody [EPR7443] (AB131272)

  • 0
  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB108629

Anti-TUBA1B antibody [EPR1333] - Loading Control

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Flow Cytometry (Intracellular) - Anti-TUBA1B antibody [EPR1333] - Loading Control (AB108629)

  • 0
  • 0
Primary Antibodies

AB230253

Anti-TIMM23/TIM23 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMM23/TIM23 antibody (AB230253)

  • 4
  • 4
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

AGK also known as acylglycerol kinase is an important enzyme involved in lipid metabolism. It is responsible for phosphorylating mono- and diacylglycerol to produce phosphatidic acid. The molecular mass of AGK is approximately 45 kDa. This enzyme is expressed in various human tissues with notable expression in tissues like the heart skeletal muscle and pancreas. The presence of AGK in these tissues indicates its significant role in energy metabolism and lipid regulation.
Biological function summary

AGK participates in the biosynthesis of phospholipids important for maintaining cell membrane integrity. It is also part of the TIM22 complex in the mitochondrial inner membrane which is important for the import and assembly of proteins. This role shows that AGK not only influences lipid metabolism but also affects mitochondrial function and structure. Its activity is critical in tissues that require high energy or have a high turnover of membrane lipids.

Pathways

AGK plays a role in both the glycerolipid and phosphatidylinositol signaling pathways. In the glycerolipid pathway AGK acts to regulate the levels of lipid intermediates which are essential for diverse cellular processes. In the phosphatidylinositol pathway AGK indirectly influences processes like cell growth and survival interacting with other proteins such as PI3K and mTOR. This connects AGK to a wider network of signals impacting cellular functions and health.

AGK is linked to mitochondrial disorders particularly Sengers syndrome which affects energy metabolism. Mutations in AGK can lead to problems in the mitochondrial function highlighting its significance in mitochondrial diseases. Furthermore AGK is associated with some cancers as its dysregulation can influence tumor progression by altering lipid signaling. The interaction of AGK with proteins such as BRAF in cancer shows how it can alter cellular proliferation and survival pathways impacting disease development and progression.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed : 15939762). Does not phosphorylate sphingosine (PubMed : 15939762). Phosphorylates ceramide (By similarity). Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol (By similarity). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (PubMed : 28712724, PubMed : 28712726). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (PubMed : 28712724, PubMed : 28712726). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (PubMed : 28712724). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (PubMed : 15939762).
See full target information AGK
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com