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AB18104

Anti-Ada protein antibody [ADA-1]

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(3 제품이 사용된 논문 )

Mouse Monoclonal ADA antibody. Suitable for IP, WB, RIA and reacts with Escherichia coli samples. Cited in 3 publications.

대체 명칭 보기

b2213, JW2201, ada, Bifunctional transcriptional activator/DNA repair enzyme Ada, Regulatory protein of adaptive response

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

ADA-1

Isotype

Unknown

Carrier free

No

Reacts with

Escherichia coli

Applications

IP, WB, RIA

applications

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보완 제품

Assay Kits

AB211093

Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric)

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric) (AB211093)

  • 5
  • 1
Primary Antibodies

AB69617

Anti-DnaK antibody [8E2/2]

Western blot - Anti-DnaK antibody [8E2/2] (AB69617)

  • 4
  • 2
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
Primary Antibodies

AB186733

Anti-Argonaute-2 antibody [EPR10411]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
보관 버퍼
Preservative: 0.02% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Ada protein also known as Ada1 plays a significant role in the bacterial adaptive response to alkylation damage. It acts as a DNA methyltransferase specifically responsible for repairing methylated bases in DNA. Ada protein has an approximate mass of 39 kilodaltons. It is expressed within many bacterial species with E. coli being a common example where its function is well-studied. Ada modulates its activity through a two-step process involving the transfer of methyl groups from damaged DNA to its own cysteine and lysine residues.
Biological function summary

Ada protein serves to directly repair methylated DNA lesions which prevents mutation and maintains genetic stability. It often functions as part of a regulatory complex that initiates the expression of other DNA repair enzymes. The Ada protein acts as both a sensor and a regulator by increasing the transcription of genes involved in the adaptive response making it a central player in the cellular defense against alkylating agents.

Pathways

Ada protein's function links closely with the DNA damage repair pathways. It primarily associates with the base excision repair (BER) pathway to correct lesions caused by endogenous and exogenous agents. Its interaction with other components such as the AlkB protein highlights its role in an interconnected network aiming to maintain the integrity of the genetic material.

Ada protein's impairment correlates with increased mutation rates and potential onset of diseases like cancer due to inadequate DNA repair mechanisms. Although primarily studied in bacterial contexts its analogy in human systems particularly related proteins such as MGMT (O6-methylguanine-DNA methyltransferase) highlights its importance in understanding tumor resistance to alkylating chemotherapy agents. Understanding Ada and similar proteins provide insights into mechanisms that safeguard cellular genomes against mutagenic threats.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme (Cys-321). Also specifically repairs the Sp diastereomer of DNA methylphosphotriester lesions by the same mechanism, although the methyl transfer occurs onto a different cysteine residue (Cys-38). Cannot demethylate the other diastereomer, Rp-methylphosphotriester. This is a suicide reaction : the enzyme is irreversibly inactivated.. The methylation of Ada by methylphosphotriesters in DNA leads to its activation as a transcriptional regulator that activates the transcription of its own gene, ada, and other alkylation resistance genes, alkA, alkB and aidB.
See full target information ada
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports. Medicine 6:101997 PubMed40054457

2025

siRNA-AGO2 complex inhibits bacterial gene translation: A promising therapeutic strategy for superbug infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Wang,Wangjian Sheng,Yu Zhou,Xudong Hang,Jiayi Zhao,Yuanyuan Gu,Xiangfeng Meng,Yuefan Bai,Weili Li,Yujing Zhang,Linlin Zhang,Jing Yu,Zhen Zhou,Xiaona Li,Haorui Sun,Yanhong Xue,Tao Xu,Ke Zen,Hong Ling,Chen-Yu Zhang,Hongkai Bi,Huan Wang

eLife 4: PubMed26402460

2015

Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria.

Applications

WB

Species

Unspecified reactive species

Ryan M McCormack,Lesley R de Armas,Motoaki Shiratsuchi,Desiree G Fiorentino,Melissa L Olsson,Mathias G Lichtenheld,Alejo Morales,Kirill Lyapichev,Louis E Gonzalez,Natasa Strbo,Neelima Sukumar,Olivera Stojadinovic,Gregory V Plano,George P Munson,Marjana Tomic-Canic,Robert S Kirsner,David G Russell,Eckhard R Podack

Carcinogenesis 12:263-8 PubMed1995192

1991

Environmental mutagens that induce the adaptive response to alkylating agents in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

P Vaughan,B Sedgwick,J Hall,J Gannon,T Lindahl
제품이 사용된 논문 모두 보기
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Product promise

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