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AB223745

Anti-ADAM12 antibody

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Rabbit Polyclonal ADAM12 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADAM12 aa 700-850.

대체 명칭 보기

MLTN, UNQ346/PRO545, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, Meltrin-alpha

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ADAM12 antibody (AB223745)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADAM12 antibody (AB223745)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for ADAM12 (green) using ab223745 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM12 antibody (AB223745)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM12 antibody (AB223745)

Paraffin-embedded human placenta tissue stained for ADAM12 using ab223745 at 1/500 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ADAM12 aa 700-850. The exact immunogen used to generate this antibody is proprietary information.

O43184

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대체품

Primary Antibodies

AB223476

Anti-ADAM12 antibody

Western blot - Anti-ADAM12 antibody (AB223476)

  • 0
  • 0
Primary Antibodies

AB115203

Anti-ADAM12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM12 antibody (AB115203)

  • 0
  • 0
Primary Antibodies

AB28747

Anti-ADAM12 antibody

  • 0
  • 0
Primary Antibodies

AB234189

Anti-PD-L2 antibody [24F.10C12]

Flow Cytometry - Anti-PD-L2 antibody [24F.10C12] (AB234189)

  • 0
  • 0
Primary Antibodies

AB39155

Anti-ADAM12 antibody - Aminoterminal end

Western blot - Anti-ADAM12 antibody - Aminoterminal end (AB39155)

  • 4
  • 3

보완 제품

Assay Kits

AB171346

Human ADAM12 ELISA Kit (MCMP)

Sandwich ELISA - Human ADAM12 ELISA Kit (MCMP) (AB171346)

  • 0
  • 0
Primary Antibodies

AB32143

Anti-STAT3 (phospho S727) antibody [E121-31]

RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] (AB32143)

  • 5
  • 7
Primary Antibodies

AB92544

Anti-LRP1 antibody [EPR3724]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-LRP1 antibody [EPR3724] (AB92544)

  • 5
  • 9
Primary Antibodies

AB228474

Anti-SLC34A2 antibody [SP322] - N-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A2 antibody [SP322] - N-terminal (AB228474)

  • 5
  • 2
Primary Antibodies

AB105194

Anti-ZIP-5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-5 antibody (AB105194)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ADAM12 also known as meltrin-alpha is a member of the ADAM (a disintegrin and metalloproteinase) family and has a mass of approximately 110 kDa. It functions primarily in the modulation of cell-cell and cell-matrix interactions due to its metalloprotease and disintegrin domains. ADAM12 protein is expressed in various tissues including muscle and placenta. Although found in many cell types it is particularly abundant during embryogenesis and development reflecting its role in these processes.
Biological function summary

The ADAM12 protein holds a role in cell signaling migration and proliferation. It is often part of complexes that interact with other extracellular matrix components influencing cell adhesion and motility. Through its protease activity ADAM12 facilitates growth factor signaling by cleaving membrane-bound precursor proteins to release active growth factors. This activity plays an important role in tissue remodeling and repair.

Pathways

The ADAM12 protein is key in the EGFR signaling pathway where it modulates the availability of epidermal growth factor (EGF) by shedding EGF-like ligands from the cell surface. This action connects ADAM12 with proteins such as ERBB receptors influencing cell division and growth. Another significant pathway is the TGF-beta signaling pathway where ADAM12's interaction with latent TGF-beta complexes can impact the bioavailability of this cytokine therefore relating to proliferation and differentiation of cells.

ADAM12 is linked to cancer progression and metastasis as its activity can positively influence tumor cell invasiveness and growth. Elevated levels of ADAM12 are observed in certain cancer types including breast and prostate cancer. Additionally it associates with metabolic disorders such as obesity where ADAM12 impacts adipogenesis and insulin sensitivity. In cancer ADAM12 interacts with proteins like matrix metalloproteinases (MMPs) to remodel the tumor microenvironment promoting cancer cell invasion.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
See full target information ADAM12
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature : PubMed38057666

2023

A human embryonic limb cell atlas resolved in space and time.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Zhang,Peng He,John E G Lawrence,Shuaiyu Wang,Elizabeth Tuck,Brian A Williams,Kenny Roberts,Vitalii Kleshchevnikov,Lira Mamanova,Liam Bolt,Krzysztof Polanski,Tong Li,Rasa Elmentaite,Eirini S Fasouli,Martin Prete,Xiaoling He,Nadav Yayon,Yixi Fu,Hao Yang,Chen Liang,Hui Zhang,Raphael Blain,Alain Chedotal,David R FitzPatrick,Helen Firth,Andrew Dean,Omer Ali Bayraktar,John C Marioni,Roger A Barker,Mekayla A Storer,Barbara J Wold,Hongbo Zhang,Sarah A Teichmann
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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