AB45041

Anti-ADAMTS12 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ADAMTS12 antibody. Suitable for WB and reacts with Mouse, African green monkey samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADAMTS12.

1 이미지

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Western blot - Anti-ADAMTS12 antibody (AB45041)

Full-length ADAMTS12 has been detected at 178 kDa by WB. However, study looking at ADAM-TS 12 expression in COS-7 cells (Gal et al. (2001) J. Biol. Chem. 276 : 17932), has detected an 83 kD COOH-terminal cleavage product.It is possible that the post-translation processing in COS-7 cells is different than that of other cells, that the epitope tag is cleaved during processing, or that the levels of the 83 kD product in other cells were below detection.

All lanes:

Western blot - Anti-ADAMTS12 antibody (ab45041) at 1/1000 dilution

Lane 1:

Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TPA)

Lane 2:

Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with IL1-alpha)

Lane 3:

Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TNF-alpha)

Predicted band size: 178 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, African green monkey

Applications

applications

Immunogen

Synthetic Peptide within Human ADAMTS12. The exact immunogen used to generate this antibody is proprietary information.

P58397

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS12 also recognized as A Disintegrin and Metalloproteinase with Thrombospondin Motifs 12 is a member of the ADAMTS family. It is a secreted enzyme with a mass of approximately 204 kDa. This protease is involved in extracellular matrix remodelling. The expression of ADAMTS12 occurs mainly in placental tissue and at lower levels in various other tissues including the lung and heart. It plays a role in processing protein substrates in the extracellular environment.

Biological function summary

The interactions with other matrix components allow ADAMTS12 to mediate matrix organization and degradation. It does not form part of a larger protein complex on its own but interacts with extracellular matrix proteins like aggrecan and versican. These interactions facilitate the breakdown of large proteoglycans aiding tissue morphogenesis and repair.

Pathways

ADAMTS12 participates in the extracellular matrix organization and degradation pathways. These pathways involve proteins such as aggrecanase and versicanase which ADAMTS12 closely relates to through its proteolytic activity. It plays a significant role in matrix remodelling which is essential for cellular migration and differentiation processes.

Alterations in ADAMTS12 expression link to osteoarthritis and cancer. In osteoarthritis increased ADAMTS12 activity contributes to cartilage degradation. In cancer its expression modifies cellular environments influencing tumor progression. It also interacts with matrix proteins like aggrecan in osteoarthritis and demonstrates associations with cancer-related proteins contributing to disease pathology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Metalloprotease that may play a role in the degradation of COMP. Also cleaves alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties.

See full target information ADAMTS12

대체 명칭 보기

UNQ1918/PRO4389, ADAMTS12, A disintegrin and metalloproteinase with thrombospondin motifs 12, ADAM-TS 12, ADAM-TS12, ADAMTS-12

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 301:108155 PubMed39761856

2025

Unveiling ADAMTS12: A key driver of bladder cancer progression via COL3A1-Mediated activation of the FAK/PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Hua Xiao,Li-Zhe Xu,Jin-Zhuo Ning,Fan Cheng

Comparative medicine 60:118-22 PubMed20412686

2010

The t(14,15) in mouse strain CBA/CaH-T(14;15)6Ca/J causes a break in the ADAMTS12 gene.

Applications

Species

Mouse

Bengi Acar-Perk,Karen Bräutigam,Regina Grunewald,Andreas Schmutzler,Christian Schem,Norbert K Arnold,Walter Jonat,Jörg Weimer

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADAMTS12 antibody

Western blot - Anti-ADAMTS12 antibody (AB45041)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise