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AB177940

Anti-ADAMTS13 antibody [EPR6132]

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal ADAMTS13 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

대체 명칭 보기

C9orf8, UNQ6102/PRO20085, ADAMTS13, A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease

2 이미지
Flow Cytometry (Intracellular) - Anti-ADAMTS13 antibody [EPR6132] (AB177940)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ADAMTS13 antibody [EPR6132] (AB177940)

Intracellular flow cytometric analysis of permeabilized A549 cells labeling ADAMTS13 with ab177940at 1/100 dilution.

Western blot - Anti-ADAMTS13 antibody [EPR6132] (AB177940)
  • WB

Supplier Data

Western blot - Anti-ADAMTS13 antibody [EPR6132] (AB177940)

All lanes:

Western blot - Anti-ADAMTS13 antibody [EPR6132] (ab177940) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HepG2 lysate at 10 µg

Predicted band size: 154 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-ADAMTS13 antibody [EPR6132] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6132

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB90786

Anti-ADAMTS13 antibody [20A5]

  • 0
  • 0
Primary Antibodies

AB28274

Anti-ADAMTS13 antibody

Western blot - Anti-ADAMTS13 antibody (AB28274)

  • 4
  • 1
Primary Antibodies

AB72335

Anti-ADAMTS13 antibody

Immunoprecipitation - Anti-ADAMTS13 antibody (AB72335)

  • 0
  • 0
Primary Antibodies

AB264161

Anti-ADAMTS13 antibody

Immunoprecipitation - Anti-ADAMTS13 antibody (AB264161)

  • 0
  • 0
Primary Antibodies

AB28273

Anti-ADAMTS13 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS13 antibody (AB28273)

  • 0
  • 0
Primary Antibodies

AB264162

Anti-ADAMTS13 antibody

Immunoprecipitation - Anti-ADAMTS13 antibody (AB264162)

  • 0
  • 0
Primary Antibodies

AB71550

Anti-ADAMTS13 antibody

  • 4
  • 1

보완 제품

Assay Kits

AB234559

Human ADAMTS13 ELISA Kit

Recombinant|SimpleStep

ELISA - Human ADAMTS13 ELISA Kit (AB234559)

  • 0
  • 0
Primary Antibodies

AB316979

Anti-Von Willebrand Factor antibody [RM1068]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [RM1068] (AB316979)

  • 0
  • 1
Primary Antibodies

AB267787

Anti-PTEN antibody [EPR22636-122]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [EPR22636-122] (AB267787)

  • 0
  • 0
Primary Antibodies

AB109190

Anti-LI Cadherin antibody [EPR3996]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (AB109190)

  • 0
  • 0
Primary Antibodies

AB85927

Anti-Cyclin C antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin C antibody (AB85927)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS13 also known as von Willebrand factor-cleaving protease (VWFCP) is a zinc-containing metalloprotease with a molecular mass of approximately 190 kDa. This protein belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family and exhibits a complex structure with specific domains including a metalloprotease and a disintegrin-like domain. ADAMTS13 is produced mainly in the liver and circulates in the blood plasma. Research shows that it primarily facilitates the cleavage of von Willebrand factor (vWF) a large multimeric protein essential for blood clotting.
Biological function summary

ADAMTS13 plays a role in regulating the size and function of von Willebrand factor (vWF) ensuring proper hemostatic balance. The enzyme prevents the accumulation of ultra-large vWF multimers which can lead to spontaneous platelet aggregation and thrombus formation. Although ADAMTS13 acts independently its function is closely linked to the dynamics of vWF in response to vascular injury. Properly functioning ADAMTS13 aids in maintaining normal blood flow by preventing unnecessary clot formation in the bloodstream.

Pathways

ADAMTS13 is critical in the coagulation and hemostatic pathways. It operates by modulating the activity of vWF which plays an essential role in platelet adhesion and aggregation forming part of the coagulation cascade. A significant relationship exists between ADAMTS13 and vWF in these pathways as the protease controls vWF multimer size directly impacting clot formation. In the context of hemostatic balance ADAMTS13 intersects with factors like thrombin and fibrinogen which further contribute to clotting processes.

ADAMTS13 deficiency or dysfunction is associated with thrombotic thrombocytopenic purpura (TTP) a rare but severe blood disorder. In TTP the reduced activity of ADAMTS13 leads to an over-accumulation of ultra-large vWF multimers resulting in excessive platelet aggregation and microvascular thrombosis. Another related condition is atypical hemolytic uremic syndrome (aHUS) where abnormal ADAMTS13 activity might exacerbate the disease pathology. Both disorders highlight the critical need for balanced ADAMTS13 function in maintaining vascular health and highlight its potential as a therapeutic target.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.
See full target information ADAMTS13
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cardiovascular medicine 9:1059124 PubMed36794234

2023

Pro-inflammatory role of Wnt/β-catenin signaling in endothelial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Kerry S Wadey,Alexandros Somos,Genevieve Leyden,Hazel Blythe,Jeremy Chan,Lawrence Hutchinson,Alastair Poole,Aleksandra Frankow,Jason L Johnson,Sarah J George

Scientific reports 8:7859 PubMed29777164

2018

mRNA treatment produces sustained expression of enzymatically active human ADAMTS13 in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Susan Liu-Chen,Brendan Connolly,Lei Cheng,Romesh R Subramanian,Zhaozhong Han
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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