AB314856

Anti-ADAMTS4 antibody [EPR24094-30]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal ADAMTS4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

KIAA0688, UNQ769/PRO1563, ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4, ADAM-TS 4, ADAM-TS4, ADAMTS-4, ADMP-1, Aggrecanase-1

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Supplier Data

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (AB314856)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 14701864, PMID : 36144730, PMID : 23319426). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1 : 180 seconds, lane 2 : 15 seconds

All lanes:

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (ab314856) at 1/1000 dilution

Lane 1:

Human hippocampus tissue lysate at 20 µg

Lane 2:

Mouse articular cartilage tissue lysate at 20 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Lane 2:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 75 kDa

false

Supplier Data

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (AB314856)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression of ADAMTS4 is upregulated in response to IFN-gamma treatment (PMID : 17606262, PMID : 20625753). Low expression : 293T (PMID : 14744861). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 14701864, PMID : 36144730, PMID : 23319426). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (ab314856) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 treated with /ml IFN-gamma for 24 hours whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 48s

Supplier Data

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (AB314856)

Blocking and diluting buffer and concentration : 5% NFDM/TBST ADAMTS4 is highly expressed in liver and brain(PMID : 24827165). Low expression : spleen, skeletal muscle. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 14701864, PMID : 36144730, PMID : 23319426). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lanes 1-3 : 15 seconds, lanes 4-6 : 6 seconds

All lanes:

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (ab314856) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Lanes 4 - 6:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 75 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24094-30

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS4 also known as aggrecanase-1 is a protein responsible for extracellular matrix degradation. It weighs approximately 90 kDa. This metalloproteinase is expressed in various tissues including cartilage and synovial tissue. It cleaves aggrecan a core protein of the proteoglycan into fragments impacting the integrity and function of the cartilage matrix. It is secreted as a latent enzyme and undergoes activation in the extracellular space.

Biological function summary

ADAMTS4 influences tissue remodeling through the breakdown of cartilage. It contributes to the disassembly of aggrecan and other matrix components influencing extracellular matrix turnover. As part of a group of similar enzymes it plays a role in proteolytic processes important for normal tissue maintenance and repair. Its actions affect the balance between matrix synthesis and degradation which is important for maintaining tissue homeostasis.

Pathways

ADAMTS4 participates in pathways such as the inflammation-related cytokine signaling and the degradation of the extracellular matrix. This protein interacts closely with ADAMTS5 contributing to matrix degradation and modulation of inflammatory processes. In these pathways the balanced activity of ADAMTS4 is essential for proper cartilage function and the resolution of inflammation in tissues.

ADAMTS4 is notably linked to osteoarthritis and rheumatoid arthritis. These conditions involve excessive cartilage breakdown where ADAMTS4 and ADAMTS5 contribute significantly to disease progression through aggrecan degradation. Understanding the activity and regulation of ADAMTS4 offers insights into potential therapeutic strategies for managing cartilage degeneration and inflammatory joint diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cleaves aggrecan, a cartilage proteoglycan, at the '392-Glu-|-Ala-393' site and may be involved in its turnover (PubMed : 10356395, PubMed : 10827174). Also cleaves COMP (PubMed : 39672391). May play an important role in the destruction of aggrecan in arthritic diseases. Could be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease.

See full target information ADAMTS4

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of orthopaedic surgery and research 19:109 PubMed38308345

2024

PDK4 inhibits osteoarthritis progression by activating the PPAR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengnan Li,Lifeng Xie,Hui Zeng,Yaohong Wu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADAMTS4 antibody [EPR24094-30]

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (AB314856)

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (AB314856)

Western blot - Anti-ADAMTS4 antibody [EPR24094-30] (AB314856)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise