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AB122280

Anti-ADAT2 antibody

3

(2 리뷰들)

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ADAT2 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADAT2 aa 1-100.

대체 명칭 보기

DEADC1, ADAT2, tRNA-specific adenosine-34 deaminase catalytic subunit ADAT2, Deaminase domain-containing protein 1

5 이미지
Immunocytochemistry - Anti-ADAT2 antibody (AB122280)
  • ICC

Unknown

Immunocytochemistry - Anti-ADAT2 antibody (AB122280)

Immunocytochemistry/immunofluorescence analysis of human cell line HeLa labelling with ab122280 at 2 μg/mL shows localization to cytosol & the Golgi apparatus.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (AB122280)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (AB122280)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ADAT2 with ab122280 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (AB122280)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (AB122280)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling ADAT2 with ab122280 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (AB122280)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (AB122280)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling ADAT2 with ab122280 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (AB122280)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (AB122280)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling ADAT2 with ab122280 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human ADAT2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6V5

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보완 제품

Proteins & Peptides

AB101153

Recombinant Human ADAT2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human ADAT2 protein (His tag N-Terminus) (AB101153)

  • 0
  • 0
Assay Kits

AB118970

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)

Biochemical assay - Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (AB118970)

  • 5
  • 3
Assay Kits

AB102500

Hydrogen Peroxide Assay Kit

Functional Studies - Hydrogen Peroxide Assay Kit (AB102500)

  • 0
  • 0
Primary Antibodies

AB33279

FITC Anti-P-Selectin (CD62P) antibody [AK-6]

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ADAT2 also known as ADAT2 deaminase plays an important role in RNA editing by converting adenosines to inosines in tRNA molecules. This deaminase has a mass of roughly 50 kDa and is widely expressed in various tissues. Its presence is important for maintaining RNA stability and accurate coding which together plays pivotal part in protein synthesis.
Biological function summary

ADAT2 works as part of the ADAT complex that includes ADAT3. This complex specifically targets anticodon regions of tRNAs. The conversion of adenosine to inosine by ADAT2 changes the reading of certain codons allowing for proper interpretation of genetic code elements important for cell function and viability. ADAT2’s activity impacts the modification process essential for accurate protein translation.

Pathways

Four important mechanisms involve ADAT2 in RNA metabolic processes. It plays an integral role in the post-transcriptional modification of RNA particularly within the tRNA maturation pathway. This pathway collaborates with proteins such as ADAT3 and their interaction governs fidelity in transcribing genetic information into proteins. The editing by ADAT2 ensures that tRNA molecules can correctly interpret codons during translation.

Four recognized conditions involve faulty ADAT2 activity: one is neurodevelopmental disorders where genetic mutations affecting its function impact brain development. Another is some types of cancer where RNA editing disruptions play part in tumorigenic processes. These diseases can involve interactions with other proteins like ADAT3 that need for proper RNA editing balance. Understanding these interactions may help in developing therapeutic strategies targeting RNA editing mechanisms.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalytic subunit of the tRNA-specific adenosine-34 deaminase complex, composed of the ADAT2 catalytic subunit and the ADAT3 regulatory subunit, which deaminates adenosine-34 (the first, also called wobble position of the anticodon) to inosine in many tRNAs. Inosine-34 allows the decoding of 3 different nucleotides at the third position of mRNA codons, as inosine is able to pair with U, C, and A. Binding of the ADAT2-ADAT3 complex to tRNA is mediated by the N-terminus of ADAT3 which rotates with respect to the catalytic domain of the complex, formed by ADAT2 and the ADAT3 C-terminal domain, to position the tRNA anticodon stem-loop correctly in the ADAT2 active site. The ADAT2-ADAT3 complex is required for radial migration of projection neurons in the developing brain cortex, and the catalytic activity of the complex is necessary for this function.
See full target information ADAT2
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell stem cell 30:300-311.e11 PubMed36764294

2023

Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA mC modification.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhang,Kijun Yoon,Daniel Y Zhang,Nam-Shik Kim,Guo-Li Ming,Hongjun Song

Evolution; international journal of organic evolution 74:2451-2464 PubMed32989734

2020

Short-term heritable variation overwhelms 200 generations of mutational variance for metabolic traits in Caenorhabditis elegans.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay M Johnson,Olivia J Smith,Daniel A Hahn,Charles F Baer
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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