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AB228606

Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free

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Rabbit Recombinant Monoclonal Adenosine phosphate antibody. Carrier free. Suitable for ELISA and reacts with Chemical samples.

대체 명칭 보기

ADP, AMP, ATP, Adenosine diphosphate, Cyclic adenosine-3閳, 5閳-monophosphate, adenosine monophosphate, adenosine triphosphate

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ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (AB228606)
  • ELISA

Unknown

ELISA - Anti-Adenosine phosphate antibody [EPR8471] - BSA and Azide free (AB228606)

This data was developed using ab140604, the same antibody clone in a different buffer formulation.50 μl of 0.1μg/ml of cAMP-SPDP-BSA was coated into 96-wells. After blocking with 1% BSA, 25 μl of 5 mg/ml of cAMP, 5'-AMP, 5'-AMP-Na2, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, or H2O and 25 μl of ab140604 at 0.5 μg/ml were added to 96-wells and incubated for 30 min on a shaker. After washing, HRP conjugated goat anti-rabbit IgG Fc specific antibody was added and incubated. After washing, TMB substrate was used to develop color.

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8471

Isotype

IgG

Carrier free

Yes

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes cAMP, AMP, ADP, and ATP only. It has less cross-reactivity with cGMP, GMP, GDP,GTP, and Adenosine.

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보완 제품

Primary Antibodies

AB215203

Anti-cleaved N-terminal GSDMD antibody [EPR20829-408]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (AB215203)

  • 5
  • 4
Primary Antibodies

AB182844

Anti-Hsp70 antibody [EPR17677]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR17677] (AB182844)

  • 0
  • 0
Assay Kits

AB113849

Luminescent ATP Detection Assay Kit

Functional Studies (Act) - Luminescent ATP Detection Assay Kit (AB113849)

  • 5
  • 6
Assay Kits

AB102536

BCA Protein Assay Kit

Functional Studies - BCA Protein Assay Kit (AB102536)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab228606 is the carrier-free version of ab140604.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine phosphate commonly referred to as adenine nucleotides includes compounds like adenosine monophosphate (AMP) adenosine diphosphate (ADP) and adenosine triphosphate (ATP). These molecules have important roles in energy transfer and storage. All types of cells express adenosine phosphates reflecting its significance in cellular activities. The molecular weight can vary depending on the number of phosphate groups: for example ATP which is the most well-known form typically has a mass of approximately 507.18 g/mol.
Biological function summary

Adenosine phosphates serve as energy currency within the cell. They do not generally exist in isolation but are part of larger complexes and biochemical structures. For instance ATP is particularly known for its role in coupling and driving endergonic reactions ensuring cells receive the necessary energy for various processes. When hydrolyzed ATP releases a significant amount of energy which is then harnessed by cellular machinery to perform tasks like biosynthesis motility and division.

Pathways

The role of adenosine phosphates is central to cellular respiration and metabolism. The oxidative phosphorylation pathway within mitochondria is a critical process involving ATP synthesis. Related proteins include various subunits of ATP synthase which play roles in converting ADP to ATP. Another relevant pathway is glycolysis where ADP and ATP are key players in the breakdown of glucose to provide energy with enzymes like phosphofructokinase aiding the transformation processes.

Its dysregulation or imbalance is linked to conditions such as mitochondrial diseases and ischemic events. Mitochondrial diseases frequently result from impaired energy production involving proteins like mitochondrial ATP synthase. Meanwhile ischemic conditions connecting to disrupted ATP supply can exacerbate symptoms or lead to further cellular damage. Understanding the role of adenosine phosphates in these disorders can guide research for potential therapeutic interventions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information Adenosine phosphate
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Product promise

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