AB325013

Anti-Adenosine Receptor A2a antibody [EPR28040-78]

RabMAb
Recombinant
20ul selling size
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Rabbit Recombinant Monoclonal Adenosine Receptor A2a antibody. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human ADORA2A expression vector containing a Myc-His tag cell pellet. (B) HEK-293T transfected with a human ADORA2B expression vector containing a Myc-His tag cell pellet. (C) HEK-293T transfected with an empty vector containing a Myc-His tag cell pellet. tissue labeling Adenosine Receptor A2a with ab325013 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a human ADORA2A expression vector containing a Myc-His tag cell pellet, no staining on (B) HEK-293T transfected with a human ADORA2B expression vector containing a Myc-His tag cell pellet and (C) HEK-293T transfected with an empty vector containing a Myc-His tag cell pellet. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Adenosine Receptor A2a with ab325013 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on human liver. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Adenosine Receptor A2a with ab325013 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on human kidney. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemical analysis of paraffin-embedded (A) SK-BR-3 (human breast adenocarcinoma epithelial cell) cell pellet. (B) NCI-H929 (human Bone B lymphocyte) cell pellet. (C) SUM149PT (human breast adenocarcinoma cell) cell pellet. (D) HeLa (human cervical adenocarcinoma epithelial cell) cell pellet. tissue labeling Adenosine Receptor A2a with ab325013 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on (A) SK-BR-3 and (B) NCI-H929, no staining on (C) SUM149PT and (C) HeLa. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemical analysis of paraffin-embedded Human corpus striatum tissue labeling Adenosine Receptor A2a with ab325013 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human corpus striatum. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Adenosine Receptor A2a with ab325013 at 1/500 (1.04 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human breast carcinoma. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28040-78

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

style

left-column

제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).

See full target information ADORA2A

대체 명칭 보기

ADORA2, ADORA2A, Adenosine receptor A2a

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Adenosine Receptor A2a antibody [EPR28040-78]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR28040-78] (AB325013)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise