Anti-Adiponectin antibody [RP23040035]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Adiponectin Antibody [RP23040035] (AB313402)

Immunofluorescent analysis of 90% confluent BM-MSCs differentiated into adipocytes fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature labeling Adiponectin with ab313402 at 5 µg/mL followd by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing cytoplasmic as well as extracellular localization. Panel e represents undifferentiated BM-MSCs showing no Adiponectin staining. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• IHC-P

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Immunohistochemistry paraffin embedded sections - Anti-Adiponectin Antibody [RP23040035] (AB313402)

Immunohistochemical analysis of paraffin-embedded human kidney tissue (right) compared to negative control without primary antibody (left) labeling Adiponectin with ab313402 at 1/100 dilution followed by an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Counter stained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS.

• WB

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Western blot - Anti-Adiponectin Antibody [RP23040035] (AB313402)

All lanes:

Western blot - Anti-Adiponectin Antibody [RP23040035] (ab313402) at 2 µg/mL

Lane 1:

Mouse adipose tissue lysate at 30 µg

Lane 2:

Human plasma at 2 µL

Lane 3:

Conditioned media from BM-MSC differentiated into adipocytes at 2 µL

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Observed band size: 26 kDa

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