AB216071

Anti-AGR2 + AGR3 antibody [EPR20164-222]

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal Anterior Gradient 2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

대체 명칭 보기

AG2, UNQ515/PRO1030, AGR2, Anterior gradient protein 2 homolog, AG-2, hAG-2, HPC8, Secreted cement gland protein XAG-2 homolog, BCMP11, PDIA18, UNQ642/PRO1272, AGR3, Anterior gradient protein 3, AG-3, AG3, hAG-3, Anterior gradient 3 homolog, Breast cancer membrane protein 11

7 이미지

Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MCF7 cell line.

Negative control : HeLa (PMID : 16551856).

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution

Lane 1:

Mouse stomach tissue lysate at 10 µg

Lane 2:

Rat stomach tissue lysate at 10 µg

Lane 3:

Human lung tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa,20 kDa

Observed band size: 17 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/2000 dilution

All lanes:

Human stomach lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa,20 kDa

Observed band size: 17 kDa,18 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Blocking/Dilution buffer : 5% NFDM/TBST.

According to the literature AGR2 is not expressed in HeLa (PMID : 16551856). Currently no published data is available regarding the expression of AGR3 in HeLa cells.

All lanes:

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa,20 kDa

Observed band size: 17 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 15 seconds; Lane 2 : 3 seconds.

All lanes:

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (ab216071) at 1/10000 dilution

Lane 1:

Human AGR2 His-tagged recombinant protein (aa21-175) at 0.01 µg

Lane 2:

Human AGR3 GST-tagged recombinant protein (aa21-166) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa,20 kDa

Observed band size: 17 kDa,42 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20164-222

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

AGR2 (anterior gradient protein 2) and AGR3 (anterior gradient protein 3) are small proteins with roles in cellular homeostasis. AGR2 has a mass of approximately 20 kDa while AGR3 is around 18 kDa. Both proteins localize in the endoplasmic reticulum (ER) and are expressed notably in epithelial tissues like the gastrointestinal tract and respiratory epithelium. AGR2 also known as PDIA17 functions as a protein disulfide isomerase which assists in protein folding. AGR3 shares structural similarities with AGR2 suggesting overlapping functions.

Biological function summary

AGR2 and AGR3 influence processes such as cell growth and repair. They are necessary for maintaining the proper folding of secreted and membrane proteins. AGR2 interacts with mucin proteins and this complex formation is important for mucin production which supports protective mucus barriers. AGR3's specific biological partners are less well-defined but it complements AGR2's functions in similar tissue contexts suggesting it might share similar interactions.

Pathways

AGR2 and AGR3 engage in pathways that link ER function to broader cellular activities. One important pathway involves the unfolded protein response (UPR) where AGR2 helps mitigate ER stress by ensuring proper protein assembly and folding. Additionally AGR2 participates in the ERK signaling pathway impacting cell proliferation and differentiation. Proteins such as ATF6 and GRP78 interact with AGR2 in these pathways indicating its role in stress response and growth signaling networks.

AGR2 and AGR3 gain attention in relation to cancer and cystic fibrosis. In cancer especially breast and prostate AGR2 overexpression correlates with tumor progression and metastasis. It interacts with proteins like MUC1 and HER2 facilitating oncogenic pathways. AGR3 while less studied shows similar trends suggesting potential involvement. In cystic fibrosis AGR2 associates with compromised mucin production and secretion causing exacerbated lung conditions. CXCL8 a mucin-related protein is seen alongside AGR2 activity in these inflammatory scenarios.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed : 23274113).

See full target information AGR2

Additional targets

AGR3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PloS one 13:e0198956 PubMed29949597

2018

Avian lungs: A novel scaffold for lung bioengineering.

Applications

Unspecified application

Species

Unspecified reactive species

Sean M Wrenn,Ethan D Griswold,Franziska E Uhl,Juan J Uriarte,Heon E Park,Amy L Coffey,Jacob S Dearborn,Bethany A Ahlers,Bin Deng,Ying-Wai Lam,Dryver R Huston,Patrick C Lee,Darcy E Wagner,Daniel J Weiss

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AGR2 + AGR3 antibody [EPR20164-222]

Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (AB216071)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

제품이 사용된 논문 (1)

Product promise