AB237622

Anti-AGXT2 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal AGXT2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AGXT2 aa 150-350.

대체 명칭 보기

AGT2, AGXT2, AGT 2, (R)-3-amino-2-methylpropionate--pyruvate transaminase, Beta-ALAAT II, Beta-alanine-pyruvate aminotransferase, D-3-aminoisobutyrate-pyruvate aminotransferase, D-AIBAT, D-beta-aminoisobutyrate-pyruvate aminotransferase

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-AGXT2 antibody (AB237622)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AGXT2 antibody (AB237622)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AGXT2 using ab237622 at a dilution of 1/233 in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT2 antibody (AB237622)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT2 antibody (AB237622)

Paraffin-embedded human kidney tissue stained for AGXT2 with ab237622 at a 1/700 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT2 antibody (AB237622)

Paraffin-embedded human liver tissue stained for AGXT2 with ab237622 at a 1/700 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human AGXT2 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q9BYV1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity greater than 95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

AGXT2 also known as alanine--glyoxylate aminotransferase 2 is an enzyme with approximately 56 kDa mass. The enzyme is expressed mainly in the liver kidney and heart. AGXT2 catalyzes the transamination reaction between alanine and glyoxylate to produce pyruvate and glycine. It is critical for the cellular detoxification processes and the regulation of amino acid metabolism.

Biological function summary

This enzyme contributes to the conversion of glyoxylate therefore preventing its accumulation which can be harmful. AGXT2 is not part of a larger protein complex but it works closely with other enzyme systems involved in amino acid metabolism. Its activity maintains the balance between glycolate and glycine levels within the body which is essential for normal cellular function.

Pathways

AGXT2 functions in both the glyoxylate and dicarboxylate metabolism pathway as well as the broader amino acid metabolism pathways. Within these pathways its role connects it to other related proteins such as Glyoxylate reductase/hydroxypyruvate reductase (GRHPR) and Lactate dehydrogenase. These interactions contribute to maintaining homeostasis in intermediary metabolism.

AGXT2 abnormalities relate closely to primary hyperoxaluria type 2 and certain cardiovascular diseases. Mutations or deficiencies in AGXT2 can result in the overproduction of oxalate leading to kidney stone formation and renal damage. Additionally reduced AGXT2 activity might influence homocysteine levels potentially linking it to cardiovascular disorders. This relationship places AGXT2 in connection with other proteins such as cystathionine beta-synthase in the context of homocysteine metabolism.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Multifunctional aminotransferase with a broad substrate specificity (PubMed : 20018850, PubMed : 23023372, PubMed : 24586340). Catalyzes the conversion of glyoxylate to glycine using alanine as the amino donor (By similarity). Catalyzes metabolism of not L- but the D-isomer of D-beta-aminoisobutyric acid to generate 2-methyl-3-oxopropanoate and alanine (PubMed : 24586340). Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate (By similarity). Can metabolize NG-monomethyl-L-arginine (NMMA), asymmetric NG,NG-dimethyl-L-arginine (ADMA) and symmetric NG,N'G-dimethyl-L-arginine (SDMA) (PubMed : 20018850, PubMed : 23023372). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure (PubMed : 20018850, PubMed : 23023372).

See full target information AGXT2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:2998 PubMed40148322

2025

Keratin-72 restricts HIV-1 infection in resting CD4 T cells by sequestering capsids in intermediate filaments.

Applications

Unspecified application

Species

Unspecified reactive species

Yang He,Meng Xu,Jiayue Ouyang,Li Zhao,Tiankui Ma,Xiaowei Zhang,Ruolin Wang,Hong Shang,Guoxin Liang

BMC anesthesiology 25:81 PubMed39966746

2025

Toxic effects of prolonged propofol exposure on cardiac development in zebrafish larvae.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojie Qian,Huizi Liu,Hanwei Wei,Jintao Liu,Xiaojun Li,Xiaopan Luo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AGXT2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AGXT2 antibody (AB237622)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT2 antibody (AB237622)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT2 antibody (AB237622)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise