AB235626

Anti-AICL antibody [EPR22061] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal AICL antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

AICL, CLECSF2, IFNRG1, CLEC2B, C-type lectin domain family 2 member B, Activation-induced C-type lectin, C-type lectin superfamily member 2, IFN-alpha-2b-inducing-related protein 1

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma tissue labeling AICL with ab221158 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Positive staining on human non-Hodgkin's lymphoma (PMID : 23929856). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221158).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U937 (human histiocytic lymphoma cell line) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling AICL with ab221158 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic and membranous staining in U937 cell line.

Negative control : HeLa (PMID : 23929856).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221158).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling AICL with ab221158 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Positive staining on human spleen (PMID : 9038101; PMID : 23929856). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221158).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

AICL was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) whole cell lysate with ab221158 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221158 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : U937 whole cell lysate 10 μg (Input).

Lane 2 : ab221158 IP in U937 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab221158 in U937 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

The expression profile observed is due to glycosylation as described in the literature (PMID : 23929856).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221158).

All lanes:

Immunoprecipitation - Anti-AICL antibody [EPR22061] (<a href='/ko/products/primary-antibodies/aicl-antibody-epr22061-ab221158'>ab221158</a>)

Predicted band size: 17 kDa

Observed band size: 26-28 kDa,45 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22061

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab235626 is the carrier-free version of ab221158.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

AICL also known as activation-induced C-type lectin is a type II transmembrane protein expressed mostly on myeloid cells especially in bone marrow. It has a molecular mass of approximately 30 kDa. AICL belongs to the C-type lectin family and is often co-expressed with other members of this family on monocytes and dendritic cells. Scientists have noted that AICL appears in both the cytoplasm and on the cell surface contributing to its interaction capabilities.

Biological function summary

AICL interacts with innate immune processes and contributes to immune cell activation. It forms a complex with immune receptors such as the NK cell receptor NKG2D modulating immune responses. AICL's ligand-binding properties facilitate engagement in pathogen recognition and response enhancing the communication between innate and adaptive immunity. These interactions underline AICL's function in monitoring cellular stress and transformation.

Pathways

AICL participates significantly in the immune response through the NKG2D signaling pathway. This pathway is essential for natural killer (NK) cell-mediated cytotoxicity linking AICL closely with NK cell activation mechanisms. Additionally AICL is involved in the cytokine signaling pathway which interacts with proteins like IL-15 that are important for immune cell proliferation and function. These pathways highlight AICL's role in regulating immune surveillance and response.

AICL has significant association with leukemia and rheumatoid arthritis. In leukemia AICL expression affects the immune cell environment influencing the progression of the disease and patient prognosis. In rheumatoid arthritis altered AICL expression connects with the inflammation process where it interacts with proteins such as MICA possibly aggravating autoimmune responses. These links highlight AICL's importance in both hematological and autoimmune disorders driving interest in its potential as a diagnostic or therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Membrane-bound protein expressed on myeloid cells which acts as a ligand to stimulate the activating receptor NKp80/KLRF1, expressed on the surface of natural killer (NK) cells. In turn, stimulates NK-cell cytotoxicity and cytokine production leading to the cytolysis of malignant CLEC2B-expressing myeloid cells.

See full target information CLEC2B

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AICL antibody [EPR22061] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

Immunocytochemistry/ Immunofluorescence - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

Immunoprecipitation - Anti-AICL antibody [EPR22061] - BSA and Azide free (AB235626)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise