Anti-AIF antibody - Mitochondrial Marker

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody - Mitochondrial Marker (AB1998)

ICC image of ab1998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1998, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody - Mitochondrial Marker (AB1998)

Immunohistochemistry of AIF in human retina with anti-AIF (IN) at 10 µg/ml.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody - Mitochondrial Marker (AB1998)

ab1998 staining AIF in human U2OS cells by Immunocytochemistry. Samples were fixed using 4% paraformaldehyde.Left image shows fixed cells labeled with ab1998 (red) and cyclophilin A (green).
Right image shows U2OS cell 6 hours after induction of apoptosis by 200 nM staurosporine.Note translocated of AIF to the nucleus upon induction of apoptosis.

Image from Varecha M et al, J Biomed Sci. 2009 Jul 6;16:59, fig 2.

• WB

Unknown

Western blot - Anti-AIF antibody - Mitochondrial Marker (AB1998)

All lanes:

Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/mL

Lane 1:

K562 cell lysate

Lane 2:

Rat heart tissue lysate

Lane 3:

Mouse heart tissue lysate

Predicted band size: 67 kDa

false

• WB

Supplier Data

Western blot - Anti-AIF antibody - Mitochondrial Marker (AB1998)

All lanes:

Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/mL

Lane 1:

NIH3T3 cell lysate at 15 µg

Lane 2:

YB2/0 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 67 kDa

false

• WB

Supplier Data

Western blot - Anti-AIF antibody - Mitochondrial Marker (AB1998)

All lanes:

Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/mL

Lane 1:

Caco-2 cell lysate at 15 µg

Lane 2:

Daudi cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 15 µg

Lane 4:

HepG2 cell lysate at 15 µg

Lane 5:

K562 cell lysate at 15 µg

Lane 6:

MCF7 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 67 kDa

false