Anti-ALDH2 antibody [EPR28021A-5]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 and MCF7 cells labelling ALDH2 with ab326874 at 1 ug/mL dilution (Green).

ab326874 staining ALDH2 in HepG2 (Positive) and MCF7 (Negative) cells cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal rabbit serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab326874 at 1µg/ml (shown in green) and ab289670 - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker - Rat IgG2a (Chimeric) (shown in pseudocolour magenta). Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

was used to counterstain tubulin at 1/ dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized SW480 cells labelling ALDH2 with ab326874 at 1 ug/mL dilution (Green).

ab326874 staining ALDH2 in SW480 wild-type and SW480-ALDH2 knockout cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal rabbit serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab326874 at 1µg/ml (shown in green) and ab289670 - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker - Rat IgG2a (Chimeric) (shown in pseudocolour magenta). Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution and ab150167 - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) pre-adsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

was used to counterstain tubulin at 1/ dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Human normal spleen tissue labeling ALDH2 with ab326874 at 1/5000 (0.1ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Minimal staining in low expressing human spleen.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Human normal kidney tissue labeling ALDH2 with ab326874 at 1/5000 (0.1ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human kidney.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Human normal liver tissue labeling ALDH2 with ab326874 at 1/5000 (0.1ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human liver.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Mouse normal kidney tissue labeling ALDH2 with ab326874 at 1/1000 (0.5ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse kidney.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Rat normal kidney tissue labeling ALDH2 with ab326874 at 1/5000 (0.1ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in rat kidney.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Rat normal liver tissue labeling ALDH2 with ab326874 at 1/5000 (0.1ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in rat liver.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Mouse normal liver tissue labeling ALDH2 with ab326874 at 1/1000 (0.5ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse liver.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Hepa 1-6, Neuro2a and primary mouse splenocytes cells labelling ALDH2 with ab326874 at 1 ug/mL dilution (Green).

ab326874 staining ALDH2 in Neuro2a, Hepa 1-6 and primary mouse splenocyte cells. The Neuro2a and Hepa 1-6 cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes, and then blocked with 1% BSA/10% normal rabbit serum/0.3M glycine in 0.1%PBS-Tween for 1h, whilst primary splenocytes were fixed with BD Cytofix/Cytoperm for 20 min before blocking with 1x PBS with 1% BSA/10µg/mL purified Rat anti-Mouse CD16/CD32/10% normal goat serum/0.3M glycine for 1h. The cells were then incubated overnight at 4°C with ab326874 at 1µg/ml (shown in green) and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control (shown in pseudocolour magenta). Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution and ab150167 - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) pre-adsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in Neuro2a and Hepa 1-6 cells fixed with 4% paraformaldehyde (10 min). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

was used to counterstain tubulin at 1/ dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Rat normal spleen tissue labeling ALDH2 with ab326874 at 1/5000 (0.1ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in low expressing rat spleen.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunohistochemical analysis of paraffin-embedded Mouse normal spleen tissue labeling ALDH2 with ab326874 at 1/1000 (0.5ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Minimal staining in low expressing mouse spleen.

The section was incubated with ab326874 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [EPR28021A-5] (AB326874)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Rat Primary Hippocampal Neurons DIV14 cells labelling ALDH2 with ab326874 at 1 ug/mL dilution (Green).

ab326874 staining ALDH2 in primary rat neurons/glia, DIV14 (prepared from E18 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDHEP) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal rabbit serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab326874 at 1µg/ml (shown in green), ab289670 - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker - Rat IgG2a (Chimeric) (shown in pseudocolour magenta) and ab104224 - Ms Anti-NeuN antibody [1B7] - Neuronal Marker (shown in pseudocolour yellow). Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution, ab150167 - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

was used to counterstain tubulin at 1/ dilution (Magenta). The Nuclear counterstain was DAPI (Blue).