AB236663

Anti-ALDH7A1 antibody

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal ALDH7A1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALDH7A1 aa 350-550.

대체 명칭 보기

ATQ1, ALDH7A1, Alpha-aminoadipic semialdehyde dehydrogenase, Alpha-AASA dehydrogenase, Aldehyde dehydrogenase family 7 member A1, Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, P6c dehydrogenase

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (AB236663)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (AB236663)

Paraffin-embedded human kidney tissue stained for ALDH7A1 using ab236663 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (AB236663)

Paraffin-embedded human liver tissue stained for ALDH7A1 using ab236663 at 1/400 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody (AB236663)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody (AB236663)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ALDH7A1 using ab236663 at a dilution of 1/133 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human ALDH7A1 aa 350-550. The exact immunogen used to generate this antibody is proprietary information.

P49419

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ALDH7A1 also known as antiquitin is an enzyme that functions in the cellular metabolism processes specifically catalyzing the oxidation of aldehydes. It has a molecular mass of about 54 kDa and can be found expressed in various tissues including liver and brain. This enzyme is part of the aldehyde dehydrogenase family which plays significant roles in detoxifying aldehydes generated by alcohol metabolism and other metabolic activities.

Biological function summary

The role of ALDH7A1 includes the breakdown of molecules within the cellular detoxification system. As a component of the lysine catabolism pathway it acts to detoxify certain aldehydes to prevent cell damage. ALDH7A1 is not known to form part of any larger protein complex but its activity is important in maintaining nitrogen balance and energy production within the body.

Pathways

Several factors dictate the participation of ALDH7A1 in metabolic functions. This protein is involved mainly in the lysine degradation pathway where it converts α-aminoadipic semialdehyde to other metabolites needed for energy production. It interacts with associated proteins such as pyridoxal phosphate for efficient function a cofactor necessary for the enzymatic action in lysine catabolism.

Defects in ALDH7A1 can lead to pyridoxine-dependent epilepsy a rare genetic disorder caused by the accumulation of toxic metabolites due to impaired enzyme function. The disorder influences neurological development and can lead to seizures without treatment. ALDH7A1 dysfunction is also linked with aldehyde dehydrogenase liver deficiency where similarities with ALDH2 — another enzyme from the same family — can influence symptoms like alcohol sensitivity and metabolic responses.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Aldehyde dehydrogenase enzyme that mediates important protective effects (PubMed : 16491085, PubMed : 20207735, PubMed : 20554659, PubMed : 21338592, PubMed : 25554827, PubMed : 31302938, PubMed : 31652343, PubMed : 38604394, PubMed : 40233740). Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes (PubMed : 16491085, PubMed : 20207735, PubMed : 21338592, PubMed : 40233740). Involved in cellular defense against hyperosmotic stress by metabolizing betaine aldehyde to betaine, an important cellular osmolyte and methyl donor (PubMed : 20207735).. Isoform 1. Involved in lysine catabolism in the brain by mediating the conversion of L-aminoadipate-semialdehyde ((S)-2-amino-6-oxohexanoate) to L-2-aminoadipate.. Isoform 2. Acts as a key inhibitor of ferroptosis both by generating membrane NADH and decreasing the level of reactive aldehydes (PubMed : 40233740). Recruited to plasma membrane in response to ferroptotic stress and phosphorylation by AMPK, generating membrane NADH to support AIFM2/FSP1 activity, an essential ferroptosis suppressor protein (PubMed : 40233740). Also directly inhibits ferroptosis by decreasing lipid peroxidation via consumption of reactive aldehydes, such as 4-hydroxynonenal (4-HNE) and malonaldehyde (PubMed : 40233740). Also acts as a regulator of cellular energy homeostasis in response to cellular energy stress, such as starvation and hypoxia, by inhibiting COPI-mediated intracellular transport, thereby reducing cellular energy consumption (PubMed : 31492851).

See full target information ALDH7A1

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell communication and signaling : CCS 18:7 PubMed31918722

2020

FOXE1 represses cell proliferation and Warburg effect by inhibiting HK2 in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weixing Dai,Xianke Meng,Shaobo Mo,Wenqiang Xiang,Ye Xu,Long Zhang,Renjie Wang,Qingguo Li,Guoxiang Cai

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALDH7A1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (AB236663)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (AB236663)

Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody (AB236663)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise