Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling ALDH7A1 with ab53278 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab53278 overnight at 4° C. Nucleus were visualized using DAPI.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

This data was developed using ab53278, the same antibody clone in a different buffer formulation.ab53278, at a 1/100 dilution, staining ALDH7A1 in paraffin embedded human liver tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

This data was developed using ab53278, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) labelling ALDH7A1 with purified ab53278 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Control : PBS only

• Flow Cyt

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Flow Cytometry - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

HepG2 cells were fixed with 100µl 4% PFA for 10 min at RT, then permeabilized with 100µl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab53278 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.

• IP

Supplier Data

Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53278).

ALDH7A1 was immunoprecipitated from 0.35 mg of human liver tissue lysate with the antibody at 1/20 dilution. Western blot was performed from the immunoprecipitated using antibody at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] (<a href='/ko/products/primary-antibodies/aldh7a1-antibody-ep1935y-ab53278'>ab53278</a>) at 1/20 dilution

Lane 1:

Human liver tissue lysate (Input) at 10 µg

Lane 2:

Human liver tissue lysate (+)

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/aldh7a1-antibody-ep1935y-ab53278'>ab53278</a> in human liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

• WB

Unknown

Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

This data was developed using ab53278, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700) at 1/50000 dilution

All lanes:

Human liver lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

All lanes:

Western blot - Anti-ALDH7A1 antibody [EP1935Y] (<a href='/ko/products/primary-antibodies/aldh7a1-antibody-ep1935y-ab53278'>ab53278</a>) at 1/10000 dilution

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ALDH7A1 antibody [EP1935Y] (<a href='/ko/products/primary-antibodies/aldh7a1-antibody-ep1935y-ab53278'>ab53278</a>) at 1/10000 dilution

Lane 1:

HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false