![Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)](https://content.abcam.com/products/images/aldosterone-antibody-epptx-r9-134-1-bsa-and-azide-free-ab251586--competitive-elisa-img78599.jpg/_jcr_content/renditions/webp-768.webp)
- cELISA
Unknown
Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)
This data was developed using ab241065, the same antibody clone in a different buffer formulation.
ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065) at 20 ng/ml.
ab241065 is specific to Aldosterone and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
![ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)](https://content.abcam.com/products/images/aldosterone-antibody-epptx-r9-134-1-bsa-and-azide-free-ab251586--elisa-img51768.jpg/_jcr_content/renditions/webp-768.webp)
- ELISA
Unknown
ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)
This data was developed using ab241065, the same antibody clone in a different buffer formulation.
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Aldosterone (0, 25, 50, 100, 200, 400, 800, 1600pg/mL) was added (25μL). This was followed by adding 50μL HRP-conjugated Aldosterone and 20-25ng/mL of ab241065 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-Aldosterone antibody [EPPTX-R9-134-1]
보완 제품
Primary Antibodies
AB281586
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [RM1007] (AB281586)](https://content.abcam.com/products/images/cd45-antibody-rm1007-ab281586--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180227.jpg)
- 5
- 3
Primary Antibodies
AB133616
BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)](https://content.abcam.com/products/images/cd4-antibody-epr6855-ab133616--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123525.jpg)
- 5
- 20
Primary Antibodies
AB52632
Anti-CD11c antibody [EP1347Y] - C-terminal
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)](https://content.abcam.com/products/images/cd11c-antibody-ep1347y-c-terminal-ab52632--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117937.jpg)
- 5
- 18
Reactivity 정보
제품 세부 정보
ab251586 is the carrier-free version of ab241065.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Aldosterone plays an essential role in regulating electrolyte balance and blood pressure by increasing sodium reabsorption and potassium excretion in the kidney's distal tubules and collecting ducts. As part of a system with other hormones like cortisol and aldosterone it modulates the body's fluid and electrolyte equilibrium. Through binding and activating the mineralocorticoid receptor aldosterone adjusts ion transport in epithelial and non-epithelial tissues impacting processes like fluid retention and blood pressure maintenance.
Pathways
Aldosterone significantly influences the renin-angiotensin-aldosterone system (RAAS) and the sodium-potassium pump. The RAAS is critical in maintaining blood pressure and fluid balance where aldosterone works closely with angiotensin II and renin. Within this pathway aldosterone promotes sodium reabsorption leading to water retention and its action is closely tied to the function of the sodium-potassium pump which further regulates cellular ionic balance and impacts systemic vascular resistance.
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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