AB322944

Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56]

BOND RX™ Validated
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal ADAM22 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56] (AB322944)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56] (AB322944)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling ADAM22 with ab322944 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human lung.
The section was incubated with ab322944 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56] (AB322944)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ADAM22 with ab322944 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human cerebrum.
The section was incubated with ab322944 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56] (AB322944)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ADAM22 with ab322944 at 1/50 (10.0 ug/ml) dilution.

Positive staining on rat cerebrum.
The section was incubated with ab322944 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28037-56

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Mouse, Rat, Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ADAM22 also known as ADAM 22 or ADAM 2 is a protein that functions mechanically as a transmembrane receptor involved in cell-cell and cell-matrix interactions. This protein does not exhibit protease activity unlike other members of the ADAM family which distinguishes its functional role. ADAM22 has a mass of approximately 100 kDa. It is expressed predominantly in the central nervous system particularly in brain tissues where it plays a significant role in neuronal signaling.

Biological function summary

ADAM22 affects the nervous system's development and function by mediating synaptic formation and maintenance. It belongs to a protein complex with LGI1 which also interacts with other proteins on the synaptic membrane facilitating communication between neurons. The LGI1-ADAM22 complex reportedly regulates ion channels and other synaptic proteins impacting neurotransmission and synaptic plasticity essential for cognitive processes.

Pathways

ADAM22 acts within the neuropharmacological and synaptogenesis pathways. It interacts closely with LGI1 and PSD95 pivotal proteins in developing synaptic signaling platforms. These pathways influence neurotransmitter release and receptor clustering fundamental for effective neural circuit function. The disruption of this pathway or its elements can lead to neurological impairments and highlight ADAM22's role in maintaining neural integrity.

ADAM22 connects to neurological conditions such as epilepsy and glioma. Mutations or deficiencies in the LGI1-ADAM22 interaction have been linked to epilepsy affecting ion channel regulatory actions. In glioma altered expression of ADAM22 may play a role in tumor progression influenced by interactions with integrins and growth factors. Therefore ADAM22's presence and regulatory actions contribute to neurological and oncological disease models.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein (PubMed : 19692335). Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.

See full target information ADAM22

대체 명칭 보기

MDC2, ADAM22, Disintegrin and metalloproteinase domain-containing protein 22, ADAM 22, Metalloproteinase-disintegrin ADAM22-3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56] (AB322944)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56] (AB322944)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ADAM22 antibody [EPR28037-56] (AB322944)

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise