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AB307628

Alexa Fluor® 488 Anti-CCL18 antibody [EPR24781-84]

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Rabbit Recombinant Monoclonal CCL18 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples.

대체 명칭 보기

AMAC1, DCCK1, MIP4, PARC, SCYA18, CCL18, C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1, CC chemokine PARC, Dendritic cell chemokine 1, Macrophage inflammatory protein 4, Pulmonary and activation-regulated chemokine, Small-inducible cytokine A18, AMAC-1, DC-CK1, MIP-4

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CCL18 antibody [EPR24781-84] (AB307628)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CCL18 antibody [EPR24781-84] (AB307628)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CCL18 with ab307628 at 1/50 (10.0 μg/ml). Negative control : no staining on human skeletal muscle. The section was incubated with ab307628 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CCL18 antibody [EPR24781-84] (AB307628)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CCL18 antibody [EPR24781-84] (AB307628)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CCL18 with ab307628 at 1/50 (10.0 μg/ml). Positive staining on human cervical carcinoma. The section was incubated with ab307628 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CCL18 antibody [EPR24781-84] (AB307628)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CCL18 antibody [EPR24781-84] (AB307628)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CCL18 with ab307628 at 1/50 (10.0 μg/ml). Positive staining on human tonsil. The section was incubated with ab307628 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24781-84

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB211649

Human CCL18 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CCL18 ELISA Kit (AB211649)

  • 5
  • 2
Primary Antibodies

AB279695

Alexa Fluor® 647 Anti-PD1 antibody [NAT105]

Recombinant|Trial Size

Flow Cytometry - Alexa Fluor® 647 Anti-PD1 antibody [NAT105] (AB279695)

  • 0
  • 0
Proteins & Peptides

AB256089

Recombinant Human CCL18 protein

SDS-PAGE - Recombinant Human CCL18 protein (AB256089)

  • 0
  • 0
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CCL18 also known as pulmonary and activation-regulated chemokine (PARC) is a chemokine protein with a molecular weight of about 8.8 kDa. It is mainly expressed in the lung but also found in lymph nodes and placenta. CCL18 is secreted by activated macrophages and dendritic cells. As a chemokine it plays a role in immune system signaling where it attracts immune cells like T lymphocytes. Researchers utilize CCL18 ELISA kits to accurately measure its levels in biological samples providing insight into its functions.
Biological function summary

CCL18 plays an important role in modulating immune responses and recruiting immune cells to sites of inflammation. It is not part of a larger complex but works closely with other components of the immune system. CCL18 influences the activity of monocytes and the differentiation of T cells which are essential for mounting effective defense against pathogens. In inflammatory conditions increased CCL18 levels suggest its importance in controlling the magnitude and duration of immune responses.

Pathways

CCL18 is involved in the immune response and inflammatory pathways. It works particularly in chemokine signaling pathways and shares functional relations with proteins like CCR3 and CCR6 which act as receptors for various chemokines. The binding of CCL18 to these receptors on targeted cells facilitates the chemotaxis of immune cells steering the immune response to appropriate areas in the body where they are needed.

CCL18 has known associations with pulmonary fibrosis and various cancers. In pulmonary fibrosis CCL18 levels correlate with disease severity and progression indicating its potential role as a biomarker. The protein interacts with other signaling molecules including collagen and matrix metalloproteinases driving the fibrotic process. In cancers especially breast cancer high CCL18 expression often corresponds to tumor progression and metastasis. These interactions highlight its relevance not only in disease progression but also as a target for therapeutic interventions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
See full target information CCL18
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com