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AB256270

Alexa Fluor® 488 Anti-CD86 antibody [OX48]

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(3 제품이 사용된 논문 )

Anti-CD86 antibody [OX48] conjugated to Alexa Fluor® 488 - suitable for flow cytometry, and reacts with rat samples. Clone [OX48] is a mouse monoclonal antibody cited in 2 publications.

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Flow Cytometry - Alexa Fluor® 488 Anti-CD86 antibody [OX48] (AB256270)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-CD86 antibody [OX48] (AB256270)

Lewis rat splenocytes treated with 10 μg/ml LPS for 3 days were stained with ab256270 (right) or mouse IgG1κ Alexa Fluor® 488 isotype (left). 10 μg/ml LPS for 3 days were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256270) or mouse IgG1κ Alexa Fluor® 488 isotype (1x 106 in 100 μl at 1 μg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD45R.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live lymphocytes.

관련 conjugated 항체와 다양한 조성의 항체 (2)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD86 antibody [OX48]

  • 578 PE

    PE Anti-CD86 antibody [OX48]

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OX48

Isotype

IgG1

Light chain 타입

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB234401

Anti-CD86 antibody [C86/2160R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/2160R] (AB234401)

  • 4
  • 2
Primary Antibodies

AB236271

Anti-CD86 antibody [C86/2160R] - BSA and Azide free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/2160R] - BSA and Azide free (AB236271)

  • 0
  • 1
Primary Antibodies

AB239075

Anti-CD86 antibody [EPR21962]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD86 antibody [EPR21962] (AB239075)

  • 0
  • 0
Primary Antibodies

AB242142

Anti-CD86 antibody [EPR22958-106]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

  • 0
  • 0
Primary Antibodies

AB243887

Anti-CD86 antibody [BLR030F] - BSA free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (AB243887)

  • 0
  • 0

보완 제품

Assay Kits

AB242239

Human CD86 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD86 ELISA Kit (AB242239)

  • 0
  • 0
Primary Antibodies

AB239230

FITC Anti-CD4 antibody [OX-35]

Lab Essentials

Flow Cytometry - FITC Anti-CD4 antibody [OX-35] (AB239230)

  • 0
  • 0
Primary Antibodies

AB238468

Anti-CD86 antibody [OX48]

Immunohistochemistry (Frozen sections) - Anti-CD86 antibody [OX48] (AB238468)

  • 5
  • 1
Primary Antibodies

AB64693

Anti-CD206 antibody

BOND RX™ Validated

Western blot - Anti-CD206 antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB283654

Anti-CD68 antibody [EPR23917-164]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size|Advanced Validation

Flow Cytometry - Anti-CD68 antibody [EPR23917-164] (AB283654)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information Cd86
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

World journal of stem cells 16:1022-1046 PubMed39734479

2024

Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance the CD74/nuclear factor-kappaB pathway in cardiac allograft.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Gang He,Xin-Xin Wu,Si Li,Dan Yan,Gao-Peng Xiao,Fu-Gang Mao

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 30:e13562 PubMed38279604

2024

Cosentyx alleviates psoriasis-induced podocyte injury by inhibiting the tlr/nf-κb signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Aixue,Wei Feng,Zhang Huanhuan,M Xixing,L Yanling

CNS neuroscience & therapeutics 27:1504-1517 PubMed34492157

2021

FBXO10 prevents chronic unpredictable stress-induced behavioral despair and cognitive impairment through promoting RAGE degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiacen Li,Qingcui Zeng,Wenjie Su,Menglong Song,Min Xie,Lei Mao
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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