Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y]

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

Immunohistochemical analysis of Paraffin-embedded Human gastric cancer tissue labelling Cytokeratin 19 with ab192643 at 1/100 dilution.

Membrane staining on human gastric cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

Immunohistochemical analysis of Paraffin-embedded Human lung cancer tissue labelling Cytokeratin 19 with ab192643 at 1/100 dilution.

Membrane staining on human lung cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

Immunohistochemical analysis of Paraffin-embedded Human glioma tissue labelling Cytokeratin 19 with ab192643 at 1/100 dilution.

Negative control : no staining on human glioma.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

Immunohistochemical analysis of Paraffin-embedded Human breast tissue labelling Cytokeratin 19 with ab192643 at 1/100 dilution.

Membrane staining on human breast cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

Immunohistochemical analysis of Paraffin-embedded Human pancreas tissue labelling Cytokeratin 19 with ab192643 at 1/100 dilution.

Membrane staining on human pancreas.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

Immunofluorescence staining of Cytokeratin 19 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using ab192643. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192643 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150120) at 2 μg/ml (shown in pseudo-color red).

Nuclear DNA was labeled in blue with DAPI.

This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

Immunofluorescence staining of Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel) using ab192643. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192643 at 1/500 dilution (shown in green) and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

Flow Cytometry analysis of Cytokeratin-19 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab192643 (red line).

The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192643, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.