AB245911

Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602]

Name: Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602]
Brand: Abcam Limited
SKU: AB245911
Rating: 5 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal H3 acetyl K27 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, Flow Cyt, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

대체 명칭 보기

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27ac

5 이미지

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

ab245911 staining Histone H3 (acetyl K27) in HeLa +/- Trichostatin A (500 ng/ml for 4h). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab245911 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^®594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry analysis of paraffin-embedded sections human liver cancer labeling Histone H3 (acetyl K27) with ab245911 at 1/100 dilution, incubated with ab245911 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Nuclear staining on human liver cancer.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry analysis of paraffin-embedded sections human colon labeling Histone H3 (acetyl K27) with ab245911 at 1/100 dilution, incubated with ab245911 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Nuclear staining on human colon.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

ab245911 at 1/500 staining Histone H3 (acetyl K27) in NIH/3T3 (mouse embryonic fibroblast) followed by ab150081 at 1/1000 dilution. The cells were fixed with 4% formaldehyde and permeabilized with 0.1% Triton X-100. ab195889 at 1/200 was used as the counterstain antibody. Nuclear DNA was labelled with DAPI (shown in blue). Confocal image showing nuclear staining in NIH/3T3 cells treated with Trichostatin A (500 ng/ml for 4h) (shown in green). The counterstain was observed with red. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry analysis of paraffin-embedded sections mouse lung labeling Histone H3 (acetyl K27) with ab245911 at 1/100 dilution, incubated with ab245911 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Nuclear staining on mouse lung.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP16602

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse, Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Binds K27ac alone and also when S28 is phosphorylated.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "This product gave a positive signal in Trichostatin A (500ng/ml 4h) treated HeLa fixed with 4% formaldehyde (10 min).", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H3C1 acetyl K27

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 27:110668 PubMed39246442

2024

Multiplex imaging of localized prostate tumors reveals altered spatial organization of AR-positive cells in the microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Çiğdem Ak,Zeynep Sayar,Guillaume Thibault,Erik A Burlingame,M J Kuykendall,Jennifer Eng,Alex Chitsazan,Koei Chin,Andrew C Adey,Christopher Boniface,Paul T Spellman,George V Thomas,Ryan P Kopp,Emek Demir,Young Hwan Chang,Vasilis Stavrinides,Sebnem Ece Eksi

Cell reports 43:114054 PubMed38578832

2024

14-3-3 binding motif phosphorylation disrupts Hdac4-organized condensates to stimulate cardiac reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Ienglam Lei,Shuo Tian,Wenbin Gao,Yijing Guo,Zhaokai Li,Ziad Sabry,Paul Tang,Y Eugene Chen,Zhong Wang

Frontiers in medicine 9:897800 PubMed35911406

2022

Establishment of the reproducible branch retinal artery occlusion mouse model and intravital longitudinal imaging of the retinal CX3CR1-GFP cells after spontaneous arterial recanalization.

Applications

Unspecified application

Species

Unspecified reactive species

Jehwi Jeon,Sang-Hoon Kim,Eunji Kong,Soo Jin Kim,Jee Myung Yang,Joo Yong Lee,Junyeop Lee,You-Me Kim,Pilhan Kim

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245911)

관련 conjugated 항체와 다양한 조성의 항체 (10)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise