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AB239270

Alexa Fluor® 488 Anti-ICAM3 antibody [MEM-171]

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Anti-ICAM3 antibody [MEM-171] conjugated to Alexa Fluor® 488 - suitable for flow cytometry, and reacts with human samples. Clone [MEM-171] is a mouse monoclonal antibody.

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Flow Cytometry - Alexa Fluor® 488 Anti-ICAM3 antibody [MEM-171] (AB239270)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-ICAM3 antibody [MEM-171] (AB239270)

Flow cytometric analysis of human peripheral blood cells labeling ICAM3 using ab239270. Surface staining. Gated on leukocytes and platelets.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-171

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ICAM3 protein. The exact immunogen used to generate this antibody is proprietary information.

P32942

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대체품

Primary Antibodies

AB10804

Anti-ICAM3 antibody [ICAM3.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (AB10804)

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Primary Antibodies

AB108618

Anti-ICAM3 antibody [EPR3995]

RabMAb|Recombinant

Western blot - Anti-ICAM3 antibody [EPR3995] (AB108618)

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Primary Antibodies

AB109405

Anti-ICAM3 antibody [EPR3994-123]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (AB109405)

  • 0
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보완 제품

Assay Kits

AB275097

Human ICAM3 ELISA Kit (CD50)

Recombinant|SimpleStep

Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (AB275097)

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Primary Antibodies

AB214944

Alexa Fluor® 647 Anti-ICAM1 antibody [EPR4776]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR4776] (AB214944)

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB308035

Anti-P97/DAP5 antibody [RP23040201]

Recombinant

  • 0
  • 0
Primary Antibodies

AB317756

Alexa Fluor® 647 Anti-IL-2 Receptor alpha antibody [EPR22816-65]

Recombinant|RabMAb

Flow Cytometry - Alexa Fluor® 647 Anti-IL-2 Receptor alpha antibody [EPR22816-65] (AB317756)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood)</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Size-exclusion chromatography
Purification 관련 사항
The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
보관 버퍼
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ICAM-3 also known as CD50 or Intercellular Adhesion Molecule 3 is a cell surface glycoprotein. It weighs approximately 120 kilodaltons. ICAM-3 is expressed majorly on leukocytes including lymphocytes monocytes and granulocytes. It is a member of the immunoglobulin superfamily that participates in cell-cell interaction particularly in immune response. By engaging with integrin receptors mainly LFA-1 ICAM-3 plays a role in leukocyte adhesion and signaling.
Biological function summary

ICAM-3 contributes to the immune system’s functionality by mediating intercellular adhesion and cellular signaling. It acts as a ligand for the integrin LFA-1 found on leukocytes which facilitates the initial immune response. ICAM-3 is not a part of a larger complex but works closely with LFA-1 in homing and recruitment of immune cells to inflammation sites. Its interaction with LFA-1 also supports the formation of the immunological synapse an important step in T-cell activation and immune signaling.

Pathways

ICAM-3 contributes to both the immune response and leukocyte extravasation pathways. The interaction with LFA-1 places it within the context of immune cell signaling pathways impacting integrin-mediated signaling. Another pathway involving ICAM-3 is the cell adhesion pathway which is essential for maintaining proper immune surveillance and response. It collaborates with proteins like ICAM-1 in these pathways to ensure effective immune activity.

ICAM-3 is associated with conditions such as inflammatory diseases and certain autoimmune disorders. Its expression levels and binding interactions can affect the severity and progression of such diseases. In autoimmune diseases ICAM-3 interaction with LFA-1 might contribute to the aberrant immune activation observed. Additionally studies have highlighted its role in infections where viruses exploit ICAM proteins to enter or spread showing its importance in pathogenesis-related conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages (PubMed : 1448173, PubMed : 1448174, PubMed : 23775590, PubMed : 8325327, PubMed : 8459213). Is also a ligand for integrin ITGAD/ITGB2 (PubMed : 8777714). Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells (PubMed : 10721994).
See full target information ICAM3

대체 명칭 보기

CD50, Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, ICAM3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of orthopaedic surgery and research 18:892 PubMed37993925

2023

Exosomal miR-331-3p derived from chemoresistant osteosarcoma cells induces chemoresistance through autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Meng,Yun Yang,Wei Feng,Penglei Ma,Rui Bai
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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