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AB252100

Alexa Fluor® 488 Anti-KAT1 / HAT1 antibody [EPR18775]

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Rabbit Recombinant Monoclonal KAT1 / HAT1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

대체 명칭 보기

KAT1, HAT1, Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, Histone methacryltransferase HAT1

1 이미지
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KAT1 / HAT1 antibody [EPR18775] (AB252100)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KAT1 / HAT1 antibody [EPR18775] (AB252100)

ab252100 staining KAT1 / HAT1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab252100 at 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alphaTubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18775

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB204536

Acetyltransferase Activity Assay Kit (Fluorometric)

Functional Studies - Acetyltransferase Activity Assay Kit (Fluorometric) (AB204536)

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Proteins & Peptides

AB93687

Recombinant Human KAT1 / HAT1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human KAT1 / HAT1 protein (His tag N-Terminus) (AB93687)

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Assay Kits

AB133099

Histone Acetyltransferase Inhibitor Screening Assay Kit

Functional Studies - Histone Acetyltransferase Inhibitor Screening Assay Kit (AB133099)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa fixed with 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein KAT1 also known as HAT1 (Histone Acetyltransferase 1) functions as an enzyme responsible for acetylating newly synthesized histone proteins. It has a mass of approximately 46 kDa. KAT1 is mostly expressed in the nucleus of cells where it modifies histones by adding acetyl groups to the N-terminal lysines a process essential for chromatin modification. The enzyme is important for facilitating transcriptional activation by altering chromatin structure and is widely observed in various eukaryotic organisms.
Biological function summary

KAT1 acts as a part of the histone acetyltransferase complex which plays a major role in regulating gene expression. By acetylating histone proteins it reduces chromatin compaction and allows gene transcription machinery access to DNA. This alteration can significantly influence cellular processes such as cell cycle regulation and DNA repair. As KAT1 interacts with several protein partners including other acetylation enzymes it serves as an important player in managing chromatin dynamics and maintaining genomic integrity.

Pathways

The role of KAT1 extends to involvement in the cell cycle and DNA damage repair pathways. In the cell cycle pathway KAT1 facilitates transcription required for progression through various phases of the cycle. In DNA damage response pathways KAT1 contributes to repairing DNA lesions by modifying chromatin to allow repair machinery access. It interacts with proteins such as p300 and CBP which are also acetyltransferases to modulate these pathways effectively and ensure cellular responses to DNA insults are adequate.

KAT1's function connects with cancer and neurodegenerative diseases due to its role in gene regulation and DNA repair. In cancer aberrant acetylation by KAT1 can lead to unregulated cell division and tumor formation while in neurodegenerative disorders impaired KAT1 activity might contribute to neuronal damage and death due to faulty DNA repair. The protein interacts with p53 another key player in both pathways impacting disease progression by modulating tumor suppressor functions and ensuring cellular stability under stress.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair (PubMed : 20953179, PubMed : 23653357, PubMed : 31278053, PubMed : 32081014). Coordinates histone production and acetylation via H4 promoter binding (PubMed : 31278053). Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac) (PubMed : 11585814, PubMed : 22615379). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression (PubMed : 31278053). Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks (PubMed : 23653357). In addition, acetylates other substrates such as chromatin-related proteins (PubMed : 32081014). Also acetylates RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation (PubMed : 31812350). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as 2-methylpropenoyl-CoA (methacryl-CoA), and is able to mediate histone methacrylation (PubMed : 34961760).. (Microbial infection) Contributes to hepatitis B virus (HBV) replication by acetylating histone H4 at the sites of 'Lys-5' and 'Lys-12' on the covalently closed circular DNA (cccDNA) minichromosome leading to its accumulation within the host cell.
See full target information HAT1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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