AB195179

Alexa Fluor® 488 Anti-p75 NGF Receptor antibody [EP1039Y]

Name: Alexa Fluor® 488 Anti-p75 NGF Receptor antibody [EP1039Y]
Brand: Abcam Limited
SKU: AB195179
Rating: 3 (2 reviews)

RabMAb
Recombinant
각 태그의 뜻

(2 리뷰들)

(1 출판물)

Rabbit Recombinant Monoclonal p75 NGF Receptor antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

1 이미지

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-p75 NGF Receptor antibody [EP1039Y] (AB195179)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-p75 NGF Receptor antibody [EP1039Y] (AB195179)

ab195179 staining p75 NGF Receptor in NGF-differentiated PC-12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195179 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

This product gave a positive signal in 4% formaldehyde (10 min) fixed NGF-differentiated PC-12 cells under the same testing conditions.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1039Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Store in the dark

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138). Together with BFAR negatively regulates NF-kappa-B and JNK-related signaling pathways (PubMed : 22566094).

See full target information NGFR

대체 명칭 보기

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

International journal of molecular sciences 23: PubMed36361868

2022

CNS Pericytes Modulate Local T Cell Infiltration in EAE.

Applications

Unspecified application

Species

Unspecified reactive species

Kathrin Koch,Maren Lindner,Ann-Katrin Fleck,Marie Liebmann,Melanie Eschborn,Lisa Zondler,Rodrigo Diéguez-Hurtado,Ralf H Adams,Gerd Meyer Zu Hörste,Alexander Zarbock,Tanja Kuhlmann,Heinz Wiendl,Luisa Klotz

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-p75 NGF Receptor antibody [EP1039Y]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-p75 NGF Receptor antibody [EP1039Y] (AB195179)

관련 conjugated 항체와 다양한 조성의 항체 (9)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise