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AB323683

Alexa Fluor® 488 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker

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Rat Monoclonal Sca1 / Ly6A/E antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples.
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Flow Cytometry - Alexa Fluor® 488 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker (AB323683)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker (AB323683)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab323683 (right) or Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323683 or Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with c-Kit.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

Flow Cytometry - Alexa Fluor® 488 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker (AB323683)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker (AB323683)

Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab323683 (right) or Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323683 or Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100μl at 0.04 μg/ml (1/12500)) for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

관련 conjugated 항체와 다양한 조성의 항체 (3)

  • Unconjugated

    Anti-Ly-6A/E (Sca-1) antibody [E13 161-7] - Hematopoietic Stem Cell Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker

  • 519 FITC

    FITC Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker

주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

E13 161-7

Isotype

IgG2a

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500 - 1/12500", "FlowCyt-species-notes": "<p></p>" } } }

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

T-cell activation.
See full target information Ly6a

대체 명칭 보기

Ly6, Ly6a, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, T-cell-activating protein, SCA-1, TAP

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com