Alexa Fluor® 488 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker
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- Flow Cyt
Lab
Flow Cytometry - Alexa Fluor® 488 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker (AB323683)
Flow cytometry staining of C57 BL/6 mouse splenocytes with ab323683 (right) or Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323683 or Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with c-Kit.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
- Flow Cyt
Lab
Flow Cytometry - Alexa Fluor® 488 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker (AB323683)
Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab323683 (right) or Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323683 or Recombinant Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100μl at 0.04 μg/ml (1/12500)) for 30min on ice.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
관련 conjugated 항체와 다양한 조성의 항체 (3)
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Anti-Ly-6A/E (Sca-1) antibody [E13 161-7] - Hematopoietic Stem Cell Marker
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker
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519 FITC
FITC Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker
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Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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