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AB197713

Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

4

(4 리뷰들)

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(11 제품이 사용된 논문 )

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker - Alexa Fluor® 488 conjugated (ab197713) is a rabbit recombinant monoclonal antibody detecting Sodium Potassium ATPase in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
1 이미지
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB197713)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB197713)

ab197713 staining Sodium Potassium ATPase in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197713 at a 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

관련 conjugated 항체와 다양한 조성의 항체 (13)

  • Unconjugated

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

  • Mouse version

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric) - BSA and Azide free

  • Rat version

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric) - BSA and Azide free

  • Rat version

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Rat IgG2a (Chimeric)

  • Mouse version

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Mouse IgG1 (Chimeric)

  • Carrier free

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free

  • 660 APC

    APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • Biotin

    Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • HRP

    HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

  • 578 PE

    PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1845Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB58475

Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control (AB58475)

  • 5
  • 1
Primary Antibodies

AB353

Anti-Sodium Potassium ATPase antibody - Plasma Membrane Loading Control

Lab Essentials

  • 0
  • 0

보완 제품

Assay Kits

AB324503

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular

SimpleStep

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

  • 0
  • 0
Primary Antibodies

AB206878

Alexa Fluor® 555 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB206878)

  • 0
  • 0
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
Primary Antibodies

AB194719

Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB194719)

  • 5
  • 1
Primary Antibodies

AB275738

Alexa Fluor® 488 Anti-CD11c antibody [EP1347Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD11c antibody [EP1347Y] (AB275738)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

What is this antibody validated in?
Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab197713) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EP1845Y is also available pre-conjugated to a variety of labels for your convenience – Anti-Sodium Potassium ATPase Alexa Fluor® 488 [EP1845Y] - Plasma Membrane Marker (ab197713).

Other related products
We have a range of other formats of antibody clone [EP1845Y] also available for your convenience: ab76020, Carrier free - ab167390, HRP - ab185065, Alexa Fluor® 488 - ab197713, Biotin - ab198366, Alexa Fluor® 647 - ab198367, PE - ab209299, Alexa Fluor® 405 - ab210143, Alexa Fluor® 555 - ab274883, ab283318, Carrier free - ab283340, Carrier free - ab283341, ab283345, APC - ab314288

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.
Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).
See full target information ATP1A1

대체 명칭 보기

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1
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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록and refine your search

Development (Cambridge, England) 151: PubMed39431301

2024

The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Simonin,Gehenna Lobo Guerrero,Sabine Bardin,Ram Venkata Gannavarapu,Denis Krndija,Joseph Boyd,Stephanie Miserey,Danijela Matic Vignjevic,Bruno Goud

The Journal of biological chemistry 300:107569 PubMed39009342

2024

Glycogen myophosphorylase loss causes increased dependence on glucose in iPSC-derived retinal pigment epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Basudha Basu,Magdalena Karwatka,Becky China,Martin McKibbin,Kamron Khan,Chris F Inglehearn,John E Ladbury,Colin A Johnson

Genes & diseases 11:101079 PubMed38560501

2024

CYP3A5 unexpectedly regulates glucose metabolism through the AKT-TXNIP-GLUT1 axis in pancreatic cancer.

Applications

Unspecified application

Species

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Ming Shao,Qingfei Pan,Haiyan Tan,Jing Wu,Ha Won Lee,Andrew D Huber,William C Wright,Ji-Hoon Cho,Jiyang Yu,Junmin Peng,Taosheng Chen

Cell reports 42:113293 PubMed37858468

2023

Single-cell and spatial analyses reveal a tradeoff between murine mammary proliferation and lineage programs associated with endocrine cues.

Applications

Unspecified application

Species

Unspecified reactive species

G Kenneth Gray,Nomeda Girnius,Hendrik J Kuiken,Aylin Z Henstridge,Joan S Brugge

Nature 606:797-803 PubMed35705814

2022

Androgen receptor blockade promotes response to BRAF/MEK-targeted therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher P Vellano,Michael G White,Miles C Andrews,Manoj Chelvanambi,Russell G Witt,Joseph R Daniele,Mark Titus,Jennifer L McQuade,Fabio Conforti,Elizabeth M Burton,Matthew J Lastrapes,Gabriel Ologun,Alexandria P Cogdill,Golnaz Morad,Peter Prieto,Alexander J Lazar,Yanshuo Chu,Guangchun Han,M A Wadud Khan,Beth Helmink,Michael A Davies,Rodabe N Amaria,Jeffrey J Kovacs,Scott E Woodman,Sapna Patel,Patrick Hwu,Michael Peoples,Jeffrey E Lee,Zachary A Cooper,Haifeng Zhu,Guang Gao,Hiya Banerjee,Mike Lau,Jeffrey E Gershenwald,Anthony Lucci,Emily Z Keung,Merrick I Ross,Laura Pala,Eleonora Pagan,Rossana Lazcano Segura,Qian Liu,Mikayla S Borthwick,Eric Lau,Melinda S Yates,Shannon N Westin,Khalida Wani,Michael T Tetzlaff,Lauren E Haydu,Mikhila Mahendra,XiaoYan Ma,Christopher Logothetis,Zachary Kulstad,Sarah Johnson,Courtney W Hudgens,Ningping Feng,Lorenzo Federico,Georgina V Long,P Andrew Futreal,Swathi Arur,Hussein A Tawbi,Amy E Moran,Linghua Wang,Timothy P Heffernan,Joseph R Marszalek,Jennifer A Wargo

Scientific reports 12:6261 PubMed35428832

2022

The lactate sensor GPR81 regulates glycolysis and tumor growth of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shota Ishihara,Kenji Hata,Katsutoshi Hirose,Tatsuo Okui,Satoru Toyosawa,Narikazu Uzawa,Riko Nishimura,Toshiyuki Yoneda

Thyroid : official journal of the American Thyroid 29:1485-1498 PubMed31310151

2019

Dimerization of the Sodium/Iodide Symporter.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca J Thompson,Alice Fletcher,Katie Brookes,Hannah Nieto,Mohammed M Alshahrani,Jonathan W Mueller,Nicholas H F Fine,David J Hodson,Kristien Boelaert,Martin L Read,Vicki E Smith,Christopher J McCabe

The Journal of clinical investigation 129:1129-1151 PubMed30741721

2019

PTEN-induced partial epithelial-mesenchymal transition drives diabetic kidney disease.

Applications

ICC/IF

Species

Unspecified reactive species

Yajuan Li,Qingsong Hu,Chunlai Li,Ke Liang,Yu Xiang,Heidi Hsiao,Tina K Nguyen,Peter K Park,Sergey D Egranov,Chandrashekar R Ambati,Nagireddy Putluri,David H Hawke,Leng Han,Mien-Chie Hung,Farhad R Danesh,Liuqing Yang,Chunru Lin

Virology journal 15:187 PubMed30509291

2018

Conserved methionine 165 of matrix protein contributes to the nuclear import and is essential for influenza A virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Petra Švančarová,Tatiana Betáková

Nature communications 9:4234 PubMed30315276

2018

Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Buskin,Lili Zhu,Valeria Chichagova,Basudha Basu,Sina Mozaffari-Jovin,David Dolan,Alastair Droop,Joseph Collin,Revital Bronstein,Sudeep Mehrotra,Michael Farkas,Gerrit Hilgen,Kathryn White,Kuan-Ting Pan,Achim Treumann,Dean Hallam,Katarzyna Bialas,Git Chung,Carla Mellough,Yuchun Ding,Natalio Krasnogor,Stefan Przyborski,Simon Zwolinski,Jumana Al-Aama,Sameer Alharthi,Yaobo Xu,Gabrielle Wheway,Katarzyna Szymanska,Martin McKibbin,Chris F Inglehearn,David J Elliott,Susan Lindsay,Robin R Ali,David H Steel,Lyle Armstrong,Evelyne Sernagor,Henning Urlaub,Eric Pierce,Reinhard Lührmann,Sushma-Nagaraja Grellscheid,Colin A Johnson,Majlinda Lako
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