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AB320072

Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

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Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to Alexa Fluor® 488. Mitochondrion marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

대체 명칭 보기

KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20

1 이미지
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB320072)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB320072)

ab320072 staining TOMM20 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab320072 at 0.2 ug/ml (shown in Green). ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647) (shown in magenta) was used at 2 ug/ml for structural counterstaining.

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This product also work with 100% methanol (5 min) fixation under the same testing conditions.

관련 conjugated 항체와 다양한 조성의 항체 (10)

  • Unconjugated

    Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • Carrier free

    Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free

  • 660 APC

    APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • HRP

    HRP Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 578 PE

    PE Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15581-54

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB109721

Complex I Enzyme Activity Microplate Assay Kit (Colorimetric)

Functional Studies - Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (AB109721)

  • 5
  • 5
Primary Antibodies

AB195020

Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020)

  • 4
  • 5
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Lab Essentials|KO Validated

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.2 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins (PubMed : 40080546). Together with TOMM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (PubMed : 18331822). The TOM complex associates with the ion channel VDAC2 and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (PubMed : 40080546). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).
See full target information TOMM20
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com