Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (AB317529)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human placental trophoblast.The section was incubated with ab317529 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (AB317529)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution.

Positive staining on capillaries of human cerebrum.The section was incubated with ab317529 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (AB317529)

Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution.

Cytoplasmic staining on human stomach cancer.The section was incubated with ab317529 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (AB317529)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in K-562 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (AB317529)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution.

Positive staining on capillaries of mouse cerebrum. The section was incubated with ab317529 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR20584] (AB317529)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transferrin Receptor with ab317529 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in Raw 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).