AB320065

Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific

BOND RX™ Validated
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Anti-Tau Alzheimer’s Disease antibody [GT-38] conjugated to Alexa Fluor® 555 - suitable for IHC-Fr, and reacts with human samples. Clone [GT-38] is a mouse monoclonal antibody.

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

Negative immunofluorescence staining of Tau Alzheimer’s Disease in a section of frozen human normal brain.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND^TM. The section was incubated at room temperature for 1 hour with ab320065 at 1/250 dilution. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

Immunofluorescence staining of Tau Alzheimer’s Disease in a section of frozen human Alzheimer brain.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BOND^TM. The section was incubated at room temperature for 1 hour with ab320065 at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

GT-38

Isotype

IgG1

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Carrier free

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "<p></p>" } } }

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제품 세부 정보

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tau protein also known as MAPT (Microtubule-Associated Protein Tau) plays a mechanical role in stabilizing microtubules in neuronal axons. The human tau protein has several isoforms generally weighing between 45 to 65 kDa. Tau is predominantly expressed in neurons of the central nervous system but can also be found in some non-neuronal cells. In neurons tau binds to tubulin and facilitates the polymerization and stability of microtubules which are essential for maintaining cellular structure and transport functions.

Biological function summary

Tau protein impacts the dynamics of the neuronal cytoskeleton. Tau is part of the intracellular transport system where it facilitates the movement of organelles and proteins along axons. This function is essential for neuron communication and resilience. Tau interacts with and regulates microtubule-associated complexes ensuring efficient intracellular transport. Its role in cytoskeletal integrity strongly influences cell signaling pathways critical for synaptic plasticity and neuronal survival.

Pathways

Tau protein plays a significant part in cellular transport and signal transduction pathways. One key pathway is the MAPK signaling pathway where tau's phosphorylation state influences neuronal response to external stimuli. Additionally tau is involved in the PI3K/AKT pathway affecting cell growth and survival. Within these pathways tau's phosphorylation is regulated by kinases including GSK-3β and CDK5 while phosphatases like PP2A dephosphorylate tau to maintain its functional state.

Dysfunctional tau is strongly linked to neurodegenerative diseases particularly Alzheimer's disease (AD) and frontotemporal dementia (FTD). In AD hyperphosphorylated tau forms neurofibrillary tangles which disrupt neuronal function and promote cell death. This process often involves other proteins such as beta-amyloid which exacerbate tau's pathological aggregation. Understanding tauopathy mechanisms has guided the research and development of anti-tau antibodies like anti-GT3 and anti-conformation antibodies as therapeutic strategies against tau-related pathologies in neurodegenerative disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Big tau

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

Immunohistochemistry (Frozen sections) - Alexa Fluor® 555 Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (AB320065)

관련 conjugated 항체와 다양한 조성의 항체 (5)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise