Alexa Fluor® 594 Anti-Myc tag antibody [9E10]

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling IL-7 with ab324645 at 1/1000 (0.507 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T (shown in green) cells transfected with a mouse Interleukin-7 or a human Interleukin-7 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5 ug/ml) dilution (Magenta).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell)cell transfected with an empty expression vector containing a myc-His-tag®; HEK-293T cell transfected with a human PNPLA3(I148M) expression vector containing a myc-His-tag® and HEK-293T cells transfected with a Human PNPLA3(WT) expression vector containing a myc-His-tag® labelling PNPLA3 with ab325644 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected a human PNPLA3 (I148M) expression vectorcontaining a myc-His-tag®, showing no staining in 293T cells transfected a human PNPLA3 (WT) or an empty expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Panel A : HEK-293T cells(human embryonic kidney epithelial cell) transfected with an empty expression vector containing a myc-His-tag®;
Panel B : HEK-293T cells transfected with a human PNPLA3 (I148M) expression vector containing a myc-His-tag®;
Panel C : HEK-293T cells transfected with a Human PNPLA3 (WT) expression vector containing a myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain myc at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling groEL with ab318970 at 1/2000 (0.251 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with an E.coli K12 Chaperonin GroEL expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling OSMR with ab323715 at 1/100 (5.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human OSMR expression vector containing a Myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling AGRP with ab323797 at 1/50 (10.02 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human AGRP expression vector containing a Myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells transfected with an empty expression vector containing a myc-His-tag® 293T cells transfected with a human Aurora A (T288A) expression vector containing a myc-His-tag® 293T cells transfected with a human Aurora A (WT) expression vector containing a myc-His-tag® cells labelling Aurora kinase A (phospho T288) with ab324635 at 1/5000 (0.096 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a human Aurora A (WT) expression vector containing a myc-His-tag®.In contrast, no staining is observed in cells transfected with either the human Aurora A (T288A) mutant or an empty expression vector containing the same myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized transfected HEK-293T cells labelling Prosurfactant Protein C with ab270521 at 1/5000 (0.1166μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody as secondary at 1/1000 (2 μg/ml) dilution. ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used as a counterstain at 1/100 (5μg/ml). Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected a mouse SFTPC expression vector containing a myc-His-tag®, showing no staining in 293T cells transfected a human SFTPC expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Panel A : HEK-293T cells transfected with an empty expression vector containing a myc-His-tag®

Panel B : HEK-293T cells transfected with a mouse SFTPC expression vector containing a myc-His-tag®

Panel C : HEK-293T cells transfected with a human SFTPC expression vector containing a myc-His-tag®

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling CD163 with ab322551 at 1/5000 (0.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human CD163 expression vector containing a myc-His-tag®, showing no staining in 293T cells transfected with a human CD163L or empty expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells transfected with an empty expression vector containing a myc-His-tag^®; HEK-293T cells transfected with a mouse SFTPC expression vector containing a myc-His-tag^®; HEK-293T cells transfected with a human SFTPC expression vector containing a myc-His-tag^® cells labeling Prosurfactant Protein C with ab259667 at 1/5000 dilution following ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected a mouse SFTPC expression vectorcontaining a myc-His-tag^® and 293T cells transfected a human SFTPC expression vector containing a myc-His-tag^®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor^® 594) was used to counterstain at 1/100 dilution (Magenta). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A Virus PB2 protein with ab320835 at 1/2000 (0.248 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with an Influenza A Virus PB2 protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MERS-CoV Spike glycoprotein with ab317040 at 1/1000 (1.03 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a MERS-CoV Spike glycoprotein protein expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a SARS-CoV-2 Spike glycoprotein vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 (2 ug/ml dilution).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

ab223894 staining Myc-tag (pseudocolored in red) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223894 at 1/100 dilution and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor^® 647 conjugated control Myc-tag antibody (different clone) is pseudocolored in white.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Tau (O-GlcNAc S400) with ab320076 at 1/500 (1.02 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a human Tau 2N4R expression vector containing a myc-His-tag®, and the signal increased after treatment with Thiamet G (10 um) for 48 h. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
A : 293T (human embryonic kidney epithelial cell) cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®
B : 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 μM Thiamet G for 48 hours
C : 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag®
D : 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag® were treated with 10 μM Thiamet G for 48 hours

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HEK-293T(human embryonic kidney epithelial cell) cells transfected with an empty expression vector containing a myc-His-tag®;HEK-293T cells transfected with a human TRBC1 expression vector containing a myc-His-tag®;HEK-293T cells transfected with a human TRBC2 expression vector containing a myc-His-tag®; cells labelling with ab325497 at 1/1000 (0.507 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected a human TRBC1 expression vectorcontaining a myc-His-tag®, showing no staining in 293T cells transfected a human TRBC2 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel A : HEK-293T cells transfected with an empty expression vector containing a myc-His-tag®; Panel B : HEK-293T cells transfected with a human TRBC1 expression vector containing a myc-His-tag®; Panel C : HEK-293T cells transfected with a human TRBC2 expression vector containing a myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells transfected with an empty expression vector containing a myc-His-tag® HEK-293T cells transfected with a human Dll1 expression vector containing a myc-His-tag® HEK-293T cells transfected with a human Dll3 expression vector containing a myc-His-tag® HEK-293T cells transfected with a human Dll4 expression vector containing a myc-His-tag® cells labelling DLL1 with ab327088 at 1/1000 (0.509 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in HEK-293T cells transfected with a human Dll1 expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). A : HEK-293T cells transfected with an empty expression vector containing a myc-His-tag®. B : HEK-293T cells transfected with a human Dll1 expression vector containing a myc-His-tag®. C : HEK-293T cells transfected with a human Dll3 expression vector containing a myc-His-tag®. D : HEK-293T cells transfected with a human Dll4 expression vector containing a myc-His-tag®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged ACE2 expression vector cells labelling ACE2 with ab314771 at 1/500 (1.092 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with ACE2 protein expression vector containing a myc tag, no staining on 293T transfected with empty vector containing a myc-tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Angiogenin with ab324046 at 1/50 (9.9 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human ANG expression vector containing a Myc tage and no staining in 293T cells transfected with empty vector or mouse ANG2, ANG3, ANG4 expression vector containing a Myc tage. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling PER2 with ab325020 at 1/200 (2.46 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a mouse PER2 expression vector containing a myc-His-tag® (shown in green), and no staining in 293T cells transfected with a mouse PER1 or PER3 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab223894 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

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Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B Virus Core Antigen with ab323646 at 1/1000 (0.488 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic and nuclear staining in 293T cells transfected with a Virus wild-type Hepatitis B Virus Core Antigen expression vector containing a Myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells(human embryonic kidney epithelial cell) transfected with an empty expression vector containing a myc-His-tag®;293T cells transfected with a human Leptin receptor protein expression vector containing a myc-His-tag®. cells labelling Leptin Receptor with ab325970 at 1/100 (5.01 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a human Leptin receptor protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling PLCZ1 with ab325528 at 1/5000 (0.103 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a human PLCZ1 vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty expression vector containing a myc-His-tag®; 293T cell transfected with a human TMEM106B expression vector containing a myc-His-tag®; 293T cell transfected with a human TMEM106C expression vector containing a myc-His-tag®; 293T cells transfected with a human TMEM106A expression vector containing a myc-His-tag® cells labelling TMEM106B with ab325555 at 1/8000 (0.062 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a human TMEM106B expression vector containing a myc-His-tag®, showing no staining in 293T cells transfected with a human TMEM106C/A or empty expression vector containing a myc-His-tag®The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Panel A : 293T cells transfected with an empty expression vector containing a myc-His-tag®.

Panel B : 293T cells transfected with a human TMEM106B expression vector containing a myc-His-tag®.

Panel C : 293T cells transfected with a human TMEM106C expression vector containing a myc-His-tag®.

Panel D : 293T cells transfected with a human TMEM106A expression vector containing a myc-His-tag®.

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain Myc at 1/100 dilution (Magenta).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized
293T cells transfected with empty vector containing a myc-HA-tag®
293T cells transfected with a human NPM1(mutation) expression vector containing a myc-HA-tag®
293T cells transfected with a human wild-type NPM1 expression vector containing a myc-HA-tag®
labelling NPM1-mutated with ab326567 at 1/500 (0.966 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a human NPM1(mutation) expression vector containing a myc-HA-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells transfected with an empty expression vector containing a myc-His-tag® HEK-293T (human epithelial cell line from embryonic kidney) transfected with a human IFNL3 expression vector containing a His-Myc tag HEK-293T (human epithelial cell line from embryonic kidney) transfected with a human IFNL2 expression vector containing a His-Myc tag cells labelling with ab327709 at 1/1000 (0.505 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in HEK-293T transfected with a human IFNL3 expression vector containing a His-Myc tag(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged AGER expression vector cells labelling RAGE with ab314773 at 1/500 (1.034 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous or cytoplasmic staining in 293T cells transfected with AGER protein expression vector containing a myc tag, no staining on 293T transfected with an empty vector containing a myc-tag.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty expression vector containing a myc-His-tag® and 293T cells transfected with a human LPL protein expression vector containing a myc-His-tag® labelling with ab328031 at 1/500 (1.044 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a human LPL protein expression vectors containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain Myc at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HBV envelope L+M protein with ab322399 at 1/4000 (0.129 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Middle envelope protein expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5 ug/ml dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B Virus Surface Antigen with ab324674 at 1/2000 (0.229 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) protein, Medium (M) protein and Small (S) protein expression vector containing a myc-His-tag® respectively. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Panel A : 293T cells transfected with an empty expression vector containing a myc-His-tag®.
Panel B : 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) protein expression vector containing a myc-His-tag®.
Panel C : 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Medium (M) protein expression vector containing a myc-His-tag®.
Panel D : 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Small (S) protein expression vector containing a myc-His-tag®.

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5 ug/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HBV envelope L protein with ab322461 at 1/50 (10.12 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) envelope protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) 293T cells transfected with a mouse CGRP I expression vector containing a myc tag 293T cells transfected with a mouse CGRP II expression vector containing a myc tag cells labelling CGRP-I with ab327004 at 1/50 (10.26 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a mouse CALCA expression vector containing a myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized transfected HEK-293T cells labelling Langerin with ab283686 at 1/1000 (1.008 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody as secondary at 1/1000 (2 μg/ml) dilution. Confocal image showing positive staining in HEK-293T cells transfected with a mouse CD207/Langerin expression vector containing a myc-His-tag® (shown in green). ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) at 1/100 (5μg/ml) was used as a counterstain (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized
HEK-293T cells transfected with a mouse Pancreatic Polypeptide expression vector containing a myc-His-tag®
HEK-293T cells transfected with a rat Pancreatic Polypeptide expression vector containing a myc-His-tag®
HEK-293T cells transfected with a mouse Peptide YY/PYY expression vector containing a myc-His-tag®
HEK-293T cells transfected with a rat Peptide YY/PYY expression vector containing a myc-His-tag®
labelling with ab327422 at 1/1000 (0.514 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in HEK-293T cells transfected with a mouse Pancreatic Polypeptide expression vector containing a myc-His-tag® and HEK-293T cells transfected with a rat Pancreatic Polypeptide expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
A : HEK-293T cells transfected with a mouse Pancreatic Polypeptide expression vector containing a myc-His-tag®.
B : HEK-293T cells transfected with a rat Pancreatic Polypeptide expression vector containing a myc-His-tag®.
C : HEK-293T cells transfected with a mouse Peptide YY/PYY expression vector containing a myc-His-tag®.
D : HEK-293T cells transfected with a rat Peptide YY/PYY expression vector containing a myc-His-tag®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain Myc at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells transfected with an empty expression vector containing a myc-His-tag® and 293T cells transfected with a mouse NPPB protein expression vector containing a myc-His-tag® labelling BNP with ab328034 at 1/2000 (0.257 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a mouse NPPB protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain myc at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with empty vector containing a myc-His-tag®. 293T cells transfected with a mouse IFNL2 expression vector containing a myc-His-tag®. 293T cells transfected with a mouse IFNL3 expression vector containing a myc-His-tag®. cells labelling Interferon lambda-2 with ab326933 at 1/500 (0.974 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a mouse IFNL2 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells(human embryonic kidney epithelial cell) transfected with an empty expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-B alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-D alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-K alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-Q alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-W28 alpha chain) protein expression vector containing a Myc-His-tag® cells labelling MHC Class I H2-Kb with ab327061 at 1/500 (1.012 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a H-2 class I histocompatibility antigen(K-B alpha chain) protein expression vector containing a Myc-His-tag® (shown in green). This antibody cross-reacts with mouse K-K, K-Q and K-W28. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel A : 293T cells(human embryonic kidney epithelial cell) transfected with an empty expression vector containing a Myc-His-tag® Panel B : 293T cells transfected with a H-2 class I histocompatibility antigen(K-B alpha chain) protein expression vector containing a Myc-His-tag® Panel C : 293T cells transfected with a H-2 class I histocompatibility antigen(K-D alpha chain) protein expression vector containing a Myc-His-tag® Panel D : 293T cells transfected with a H-2 class I histocompatibility antigen(K-K alpha chain) protein expression vector containing a Myc-His-tag® Panel E : 293T cells transfected with a H-2 class I histocompatibility antigen(K-Q alpha chain) protein expression vector containing a Myc-His-tag® Panel F : 293T cells transfected with a H-2 class I histocompatibility antigen(K-W28 alpha chain) protein expression vector containing a Myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a H-2 class I histocompatibility antigen(D-B alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(D-D alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(D-P alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(D-K alpha chain) protein expression vector containing a Myc-His-tag® cells labelling MHC Class I H2-Kb with ab327061 at 1/500 (1.012 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a H-2 class I histocompatibility antigen(K-B alpha chain) protein expression vector containing a Myc-His-tag® (shown in green). This antibody cross-reacts with mouse D-D, alpha chain and D-K. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel G : 293T cells transfected with a H-2 class I histocompatibility antigen(D-B alpha chain) protein expression vector containing a Myc-His-tag® Panel H : 293T cells transfected with a H-2 class I histocompatibility antigen(D-D alpha chain) protein expression vector containing a Myc-His-tag® Panel I : 293T cells transfected with a H-2 class I histocompatibility antigen(alpha chain) protein expression vector containing a Myc-His-tag® Panel J : 293T cells transfected with a H-2 class I histocompatibility antigen(D-P alpha chain) protein expression vector containing a Myc-His-tag® Panel K : 293T cells transfected with a H-2 class I histocompatibility antigen(D-K alpha chain) protein expression vector containing a Myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells(human embryonic kidney epithelial cell) transfected with an empty expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-B alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-D alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-K alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-Q alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(K-W28 alpha chain) protein expression vector containing a Myc-His-tag® cells labelling MHC Class I H2‑Kb with ab327060 at 1/500 (1.012 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a H-2 class I histocompatibility antigen(K-B alpha chain) protein expression vector containing a Myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel A : 293T cells(human embryonic kidney epithelial cell) transfected with an empty expression vector containing a Myc-His-tag® Panel B : 293T cells transfected with a H-2 class I histocompatibility antigen(K-B alpha chain) protein expression vector containing a Myc-His-tag® Panel C : 293T cells transfected with a H-2 class I histocompatibility antigen(K-D alpha chain) protein expression vector containing a Myc-His-tag® Panel D : 293T cells transfected with a H-2 class I histocompatibility antigen(K-K alpha chain) protein expression vector containing a Myc-His-tag® Panel E : 293T cells transfected with a H-2 class I histocompatibility antigen(K-Q alpha chain) protein expression vector containing a Myc-His-tag® Panel F : 293T cells transfected with a H-2 class I histocompatibility antigen(K-W28 alpha chain) protein expression vector containing a Myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-B alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-D alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-P alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-K alpha chain expression vector containing a myc-His-tag® cells labelling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/1000 (0.501 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-B alpha chain, D-D alpha chain, alpha chain, D-P alpha chain and D-P alpha chain expression vectors containing a myc-His-tag® (shown in green), showing no staining in 293T cells transfected with an empty expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel A : 293T cells transfected with an empty expression vector containing a myc-His-tag®. Panel B : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-B alpha chain expression vector containing a myc-His-tag®. Panel C : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-D alpha chain expression vector containing a myc-His-tag®. Panel D : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, alpha chain expression vector containing a myc-His-tag®. Panel E : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-P alpha chain expression vector containing a myc-His-tag®. Panel F : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-K alpha chain expression vector containing a myc-His-tag®.

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-B alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-D alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-K alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-Q alpha chain expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-W28 alpha chain expression vector containing a myc-His-tag® cells labelling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/1000 (0.501 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a mouse H-2 class I histocompatibility antigen, D-P alpha chain, D-K alpha chain, K-B alpha chain, K-D alpha chain, K-K alpha chain, K-Q alpha chain and K-W28 alpha chain expression vectors containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel G : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-B alpha chain expression vector containing a myc-His-tag®. Panel H : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-D alpha chainexpression vector containing a myc-His-tag®. Panel I : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-K alpha chain expression vector containing a myc-His-tag®. Panel J : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-Q alpha chain expression vector containing a myc-His-tag® Panel K : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, K-W28 alpha chain expression vector containing a myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (AB223894)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a mouse H-2 class I histocompatibility antigen, L-D alpha chain (H2-L) expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q10 alpha chain (H2-Q10)expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q8 alpha chain (H2-Q8) expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q8 alpha chain (H2-Q7) expression vector containing a myc-His-tag® 293T cells transfected with a mouse H-2 class I histocompatibility antigen, alpha chain, Clone PH-2D-2 expression vector containing a myc-His-tag® cells labelling MHC Class 1 H2 Db/H2-D1 with ab326943 at 1/1000 (0.501 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a mouse H-2 class I histocompatibility antigen, L-D alpha chain (H2-L), Q10 alpha chain (H2-Q10), Q8 alpha chain (H2-Q8), Q8 alpha chain (H2-Q7) and alpha chain, Clone PH-2D-2 expression vectors containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel L : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, L-D alpha chain (H2-L) expression vector containing a myc-His-tag® Panel M : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q7 alpha chain (H2-Q7) expression vector containing a myc-His-tag® Panel N : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q8 alpha chain (H2-Q8) expression vector containing a myc-His-tag® Panel O : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, Q7 alpha chain (H2-Q7) expression vector containing a myc-His-tag® Panel P : 293T cells transfected with a mouse H-2 class I histocompatibility antigen, alpha chain, Clone PH-2D-2 expression vector containing a myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.