AB215491

Alexa Fluor® 594 Anti-TRAF2 antibody [EPR6048]

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal TRAF2 antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples. Cited in 1 publication.

대체 명칭 보기

TRAP3, TRAF2, TNF receptor-associated factor 2, E3 ubiquitin-protein ligase TRAF2, RING-type E3 ubiquitin transferase TRAF2, Tumor necrosis factor type 2 receptor-associated protein 3

1 이미지

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-TRAF2 antibody [EPR6048] (AB215491)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-TRAF2 antibody [EPR6048] (AB215491)

ab215491 staining TRAF2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215491 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6048

Isotype

IgG

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Carrier free

Reacts with

Human, Human, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TRAF2 known also as TNF Receptor-Associated Factor 2 is an adapter protein weighing approximately 56 kDa. It is expressed in many tissues such as lymph nodes spleen and thymus. This protein acts as a signal transducer playing an integral role in the activation of NF-kB and MAPK signaling pathways. TRAF2 interacts with TNF receptors and other proteins using its RING finger and zinc finger domains for ubiquitination processes.

Biological function summary

TRAF2 modulates immune responses by regulating cell survival proliferation and apoptosis. It often forms complexes with other TRAF family proteins to mediate signal transmission. These complexes control the balance between pro-apoptotic and anti-apoptotic signals impacting the regulation of immune and inflammatory responses. Its interactions are necessary for the proper function of NF-kB ensuring balanced immune activities.

Pathways

TRAF2 is a critical component within both NF-kB and JNK signaling pathways. In the NF-kB pathway TRAF2 intersects with proteins like IKK and NEMO facilitating the transcription of genes involved in immune and inflammatory responses. In JNK signaling TRAF2 influences cellular responses through regulation of apoptotic signals. By linking these pathways TRAF2 ensures a coordinated cellular response to extracellular stimuli.

TRAF2’s dysregulation is linked with autoimmune diseases and certain types of cancer. Overactivity can contribute to chronic inflammation highlighting its role in autoimmune disorders like rheumatoid arthritis. In cancer TRAF2’s involvement with TNF receptors can lead to uncontrolled cell proliferation. Proteins like cIAP1 and cIAP2 are often related to TRAF2 in these contexts influencing the progression and modulation of disease states.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

E3 ubiquitin-protein ligase that regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis (PubMed : 10346818, PubMed : 11784851, PubMed : 12917689, PubMed : 15383523, PubMed : 18981220, PubMed : 19150425, PubMed : 19810754, PubMed : 19918265, PubMed : 19937093, PubMed : 20047764, PubMed : 20064526, PubMed : 20385093, PubMed : 20577214, PubMed : 22212761). Catalyzes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, IKBKE, MLST8, RIPK1 and TICAM1 (PubMed : 23453969, PubMed : 28489822). Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases (PubMed : 15383523, PubMed : 18981220). Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain (PubMed : 11907583, PubMed : 19506082). Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR (PubMed : 15121867). In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE (PubMed : 23453969). Acts as a regulator of mTORC1 and mTORC2 assembly by mediating 'Lys-63'-linked ubiquitination of MLST8, thereby inhibiting formation of the mTORC2 complex, while facilitating assembly of the mTORC1 complex (PubMed : 28489822). Required for normal antibody isotype switching from IgM to IgG (By similarity).

See full target information TRAF2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Disease markers 2020:5074239 PubMed32952742

2020

Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Min Ma,Meng Yu,Cong Wu,Hou-Bao Huang,Ya-Wei Li,Peng Zhang,Jian-Jun Huang,Long Cheng,Gang Feng,Guo-Rong Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 594 Anti-TRAF2 antibody [EPR6048]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-TRAF2 antibody [EPR6048] (AB215491)

관련 conjugated 항체와 다양한 조성의 항체 (5)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise