AB237337

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]

Name: Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]
Brand: Abcam Limited
SKU: AB237337
Rating: 5 (1 reviews)

RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(8 제품이 사용된 논문 )

Anti-6X His tag® antibody [EPR20547] - Alexa Fluor® 647 conjugated (ab237337) is a rabbit recombinant monoclonal antibody detecting 6X His tag® in ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 이미지

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)

ab237337 staining 6X His tag^® in 293T cells transfected with 6X His tag^® with GFP tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237337 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue) and GFP is shown in green.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20547

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in 6x His tag with GFP tag transfected 293T fixed with 4% formaldehyde (10 min).</p>" } } }

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제품 세부 정보

What is this antibody validated in?
Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR20547 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® Alexa Fluor® 647 [EPR20547] (ab237337).

Other related products
We have a range of other formats of antibody clone [EPR20547] also available for your convenience: ab213204, Carrier free - ab232492, Alexa Fluor® 488 - ab237336, Alexa Fluor® 647 - ab237337, PE - ab237338, HRP - ab237339, Biotin - ab316173, APC - ab317659, FITC - ab318311

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information 6X His tag®

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine (Cambridge, Mass.) 31:170 PubMed40325370

2025

Bacteriophage-derived endolysins restore antibiotic susceptibility in β-lactam- and macrolide-resistant Streptococcus pneumoniae infections.

Applications

Unspecified application

Species

Unspecified reactive species

Niels Vander Elst,Kristine Farmen,Lisa Knörr,Lotte Merlijn,Federico Iovino

PLoS pathogens 20:e1012318 PubMed38865374

2024

Arboviruses antagonize insect Toll antiviral immune signaling to facilitate the coexistence of viruses with their vectors.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Jia,Guozhong Luo,Heran Guan,Tingting Yu,Xinyan Sun,Yu Du,Yiheng Wang,Hongyan Chen,Taiyun Wei

PLoS genetics 20:e1011127 PubMed38829907

2024

The conserved σD envelope stress response monitors multiple aspects of envelope integrity in corynebacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth M Hart,Evan Lyerly,Thomas G Bernhardt

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38383499

2024

HERV-K (HML-2) Envelope Protein Induces Mitochondrial Depolarization and Neurotoxicity via Endolysosome Iron Dyshomeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Peter W Halcrow,Darius N K Quansah,Nirmal Kumar,Joseph P Steiner,Avindra Nath,Jonathan D Geiger

Nature communications 14:1606 PubMed36959204

2023

Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotu Ma,Xiaolong Liang,Yao Li,Qingqing Feng,Keman Cheng,Nana Ma,Fei Zhu,Xinjing Guo,Yale Yue,Guangna Liu,Tianjiao Zhang,Jie Liang,Lei Ren,Xiao Zhao,Guangjun Nie

International journal of molecular sciences 23: PubMed35054983

2022

Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Anett Hudák,Gábor Veres,Annamária Letoha,László Szilák,Tamás Letoha

Nature communications 12:6161 PubMed34697321

2021

Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus.

Applications

Unspecified application

Species

Unspecified reactive species

Guha Asthagiri Arunkumar,Disha Bhavsar,Tiehai Li,Shirin Strohmeier,Veronika Chromikova,Fatima Amanat,Mehman Bunyatov,Patrick C Wilson,Ali H Ellebedy,Geert-Jan Boons,Viviana Simon,Robert P de Vries,Florian Krammer

International journal of molecular sciences 22: PubMed34069441

2021

Contribution of Syndecans to the Cellular Entry of SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Anett Hudák,Annamária Letoha,László Szilák,Tamás Letoha

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (8)

Product promise