AB325264

Alexa Fluor® 647 Anti-ATAD3A antibody [EPR27484-26]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ATAD3A antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human, Mouse samples.

2 이미지

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATAD3A antibody [EPR27484-26] (AB325264)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATAD3A antibody [EPR27484-26] (AB325264)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling ATAD3A with ab325264 at 1/50 (10.0 ug/ml) dilution (magenta).

Confocal image showing mitochondrial staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain mitochondria, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (green).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATAD3A antibody [EPR27484-26] (AB325264)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ATAD3A with ab325264 at 1/50 (10.0 ug/ml) dilution (magenta).

Confocal image showing mitochondrial staining in NIH/3T3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27484-26

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Not suitable for mouse and rat IHC-P. This antibody recognizes overexpressed human ATAD3A isoform 2 and isoform 1 (UniProt ID: Q9NVI7). It may detect endogenous levels of ATAD3 isoform 1.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATAD3A also known as ATPase family AAA domain-containing protein 3A functions as a mitochondrial membrane protein with an approximate molecular mass of 67 kDa. This protein is expressed widely in human tissues with high levels detected in the brain heart and liver. Mechanically ATAD3A facilitates interactions between the inner and outer mitochondrial membranes playing a role in mitochondrial dynamics and integrity.

Biological function summary

The maintenance of mitochondrial structure and function heavily involves ATAD3A. It forms part of a multiprotein complex that ensures the proper organization of mitochondrial nucleoids thereby regulating mitochondrial DNA replication and transcription. It also participates in maintaining the stability and distribution of mitochondrial proteins contributing to the overall energy metabolism of the cell.

Pathways

ATAD3A engages in key processes related to mitochondrial biogenesis and dynamics. Involvement in pathways like the mitochondrial DNA replication pathway and the energy production pathway highlights its role in maintaining cellular homeostasis. The protein interacts with others including DRP1 and MFN2 essential proteins that regulate mitochondrial fission and fusion processes contributing to its role in energy metabolism.

ATAD3A has associations with several conditions affecting mitochondrial function. Mutations or dysregulation in ATAD3A contribute to neurodegenerative disorders and certain types of cancer. It has been linked to Parkinson's disease due to its influence on mitochondrial homeostasis and is connected with Alzheimer's disease through its interactions with proteins like PINK1 another critical player in mitochondrial maintenance and function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level (PubMed : 17210950, PubMed : 20154147, PubMed : 22453275, PubMed : 31522117, PubMed : 37832546, PubMed : 39116259). May play an important role in mitochondrial protein synthesis (PubMed : 22453275). May also participate in mitochondrial DNA replication (PubMed : 17210950). May bind to mitochondrial DNA D-loops and contribute to nucleoid stability (PubMed : 17210950). Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis (PubMed : 22453275). Involved in mitochondrial-mediated antiviral innate immunity (PubMed : 31522117). Required to protect mitochondria from the PERK-mediated unfolded protein response : specifically inhibits the activity of EIF2AK3/PERK at mitochondria-endoplasmic reticulum contact sites, thereby providing a safe haven for mitochondrial protein translation during endoplasmic reticulum stress (PubMed : 39116259). Ability to inhibit EIF2AK3/PERK is independent of its ATPase activity (PubMed : 39116259). Also involved in the mitochondrial DNA damage response by promoting signaling between damaged genomes and the mitochondrial membrane, leading to activation of the integrated stress response (ISR) (PubMed : 37832546).

See full target information ATAD3A

대체 명칭 보기

ATPase family AAA domain-containing protein 3A, ATAD3A, Atad3, Kiaa1273, ATPase family AAA domain-containing protein 3A, AAA-ATPase TOB3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-ATAD3A antibody [EPR27484-26]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATAD3A antibody [EPR27484-26] (AB325264)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATAD3A antibody [EPR27484-26] (AB325264)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise