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AB225310

Alexa Fluor® 647 Anti-CD4 antibody [EPR19514]

5

(1 리뷰)

|

(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CD4 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 2 publications.
1 이미지
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR19514] (AB225310)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR19514] (AB225310)

IHC image of CD4 staining in a section of frozen normal mouse spleen.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225310 at 1/250 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

관련 conjugated 항체와 다양한 조성의 항체 (5)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19514

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Mouse

Applications

IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB196372

Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (AB196372)

  • 4
  • 2
Primary Antibodies

AB181724

Anti-CD4 antibody [EPR6855] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] - BSA and Azide free (AB181724)

  • 4
  • 9
Primary Antibodies

AB238798

Anti-CD4 antibody [SP35] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-CD4 antibody [SP35] - BSA and Azide free (AB238798)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB277931

Alexa Fluor® 594 Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD4 antibody [EPR6855] (AB277931)

  • 0
  • 0

보완 제품

Assay Kits

AB234569

Human CD4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
Primary Antibodies

AB305210

Alexa Fluor® 647 Anti-CD31 antibody [EPR17259]

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD31 antibody [EPR17259] (AB305210)

  • 0
  • 0
Primary Antibodies

AB313669

Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-222]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-222] (AB313669)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
See full target information Cd4

대체 명칭 보기

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

Clinical and translational medicine 15:e70123 PubMed39748197

2025

Titin gene mutations enhance radiotherapy efficacy via modulation of tumour immune microenvironment in rectum adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hengchang Liu,Jialiang Liu,Xu Guan,Zhixun Zhao,Pu Cheng,Haipeng Chen,Zheng Jiang,Xishan Wang

Advanced science (Weinheim, Baden-Wurttemberg, Ger 7:2001724 PubMed33173731

2020

Oncogenic State and Cell Identity Combinatorially Dictate the Susceptibility of Cells within Glioma Development Hierarchy to IGF1R Targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Anhao Tian,Bo Kang,Baizhou Li,Biying Qiu,Wenhong Jiang,Fangjie Shao,Qingqing Gao,Rui Liu,Chengwei Cai,Rui Jing,Wei Wang,Pengxiang Chen,Qinghui Liang,Lili Bao,Jianghong Man,Yan Wang,Yu Shi,Jin Li,Minmin Yang,Lisha Wang,Jianmin Zhang,Simon Hippenmeyer,Junming Zhu,Xiuwu Bian,Ying-Jie Wang,Chong Liu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com