Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785] (AB280968)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Collagen I with ab280968 at 1/100 dilution.
Negative control : no staining on the human cardiac muscle.

The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab280968 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785] (AB280968)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Collagen I with ab280968 at 1/100 dilution.
Negative control : no staining on the human cerebrum.

The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab280968 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785] (AB280968)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Collagen I with ab280968 at 1/100 dilution.
Positive staining on the stroma in human endometrium. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab280968 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).