Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

Immunofluorescence of Cytokeratin 14 staining in a section of formalin-fixed paraffin-embedded human skin. Positive staining on human skin. The primary antibody was incubated for 60 mins at room temperature at 1/100 (5 μg/ml) (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• ICC

Lab

Immunocytochemistry - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

ab206100 staining Cytokeratin 14 in A431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206100 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in A431 cells fixed with 100% methanol (5 min).

• ICC

PubMed

Immunocytochemistry - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

Immunocytochemistry/immunofluorescence analysis of human mammary organoids grown on chamber slides labelling Cytokeratin 8 with ab192467 (green), Cytokeratin 14 with ab206100 (red), and Actin (white). Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature and permeabilized with 0.5% Triton X-100 for 10 minutes at 4 °C. Primary antibodies incubated overnight at 4 °C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 μm. Organoids were imaged by confocal microscopy.

Rosenbluth J et al., Nat Commun, 11(1), 1711. Fig 1c.; doi: 10.1038/s41467-020-15548-7. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

Immunofluorescence of Cytokeratin 14 staining in a section of formalin-fixed paraffin-embedded mouse skin. Positive staining on mouse skin. The primary antibody was incubated for 60 mins at room temperature at 1/100 (5 μg/ml) (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

Immunofluorescence of Cytokeratin 14 staining in a section of formalin-fixed paraffin-embedded rat skin. Positive staining on rat skin. The primary antibody was incubated for 60 mins at room temperature at 1/100 (5 μg/ml) (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.