Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling EpCAM with ab237396 at 1/50 (10 μg/ml) dilution.

Membrane staining on human endometrium.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling EpCAM with ab237396 at 1/50 (10 μg/ml) dilution.

Negative control : no staining on human skeletal muscle.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling EpCAM with ab237396 at 1/50 (10 μg/ml) dilution.

Membrane staining on human colon cancer.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling EpCAM with ab237396 at 1/50 (10 μg/ml) dilution.

Membrane staining on human colon.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling EpCAM with ab237396 at 1/50 (10 μg/ml) dilution.

Negative control : no staining on human cerebrum.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

Overlay histogram showing HT-29 cells stained with ab237396 (red line).

The cells were incubated in 1x PBS/10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237396) (1x10⁶ in 100 μl at 1 μg/ml (1/500)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20532-225] (AB237396)

ab237396 staining EpCAM in HT-29 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237396 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in ). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HT-29 cells fixed with 100% methanol (5 min).