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AB307621

Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2]

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Rabbit Recombinant Monoclonal FOXP4 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples.

대체 명칭 보기

FKHLA, FOXP4, Forkhead box protein P4, Fork head-related protein-like A

4 이미지
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling FOXP4 with ab307621 at 1/500 dilution (0.1μg) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling FOXP4 with ab307621 at 1/50 (10.0 μg/ml) dilution. Confocal image showing nuclear staining in HepG2 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling FOXP4 with ab307621 at 1/50 (10.0 μg/ml) dilution. Confocal image showing nuclear staining in Hepa1-6 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling FOXP4 with ab307621 at 1/500 dilution (0.1μg) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22714-2

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB39401

Caspase-3 Assay Kit (Colorimetric)

Functional Studies - Caspase-3 Assay Kit (Colorimetric) (AB39401)

  • 0
  • 1
Primary Antibodies

AB251675

Anti-EYA4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)

  • 0
  • 0
Assay Kits

AB39412

Caspase-1 Assay Kit (Fluorometric)

Functional Studies - Caspase-1 Assay Kit (Fluorometric) (AB39412)

  • 0
  • 0
Primary Antibodies

AB121831

Anti-SAMD12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (AB121831)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FOXP4 also known as Forkhead Box P4 is a transcription factor belonging to the FOX protein family. Its molecular mass approximately 80 kDa. It is expressed in various tissues with high expression levels observed in lung kidney and prostate. The protein binds to DNA and regulates the transcription of genes involved in development and differentiation.
Biological function summary

FOXP4 plays an important role in regulating developmental processes particularly in the central nervous system and lung tissue. It often functions as part of a complex that includes other FOXP proteins like FOXP1 and FOXP2 which share a conserved DNA-binding forkhead domain. These proteins collaborate to regulate genes important for cell differentiation and organogenesis.

Pathways

The transcriptional activity of FOXP4 integrates into significant signaling pathways such as the Wnt signaling and TGF-beta pathways. These pathways govern processes like cellular growth and differentiation. FOXP4 interacts with other FOX proteins including FOXP1 to modulate gene expression patterns important for these pathways.

Research indicates that FOXP4 is associated with lung cancer and neurodevelopmental disorders. Its dysregulation can affect cellular processes linked to tumorigenesis and cognitive development. FOXP4’s involvement with other proteins such as FOXP1 highlights its role in modulating gene networks that when disrupted can contribute to disease pathogenesis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcriptional repressor that represses lung-specific expression.
See full target information FOXP4
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com