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AB320063

Alexa Fluor® 647 Anti-Myogenin antibody [F5D]

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Anti-Myogenin antibody [F5D] conjugated to Alexa Fluor® 647 - suitable for ICC/IF, and reacts with mouse, human samples. Clone [F5D] is a mouse monoclonal antibody cited in 6 publications.
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myogenin antibody [F5D] (AB320063)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myogenin antibody [F5D] (AB320063)

ab320063 staining myogenin in 48h differenciated-C2C12 cells (2% Horse Serum + 1uM Insulin for 48hr), with negative expression in undifferenciated C2C12 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab320063 at 0.5 ug/ml (shown in magenta). ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488) (shown in green) was used at 2 ug/ml for structural counterstaining.

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This product also work with 100% methanol (5 min) fixation under the same testing conditions.

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

F5D

Isotype

IgG1

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>The immunogen used to raise this antibody has 100% homology with the Rat Myogenin protein. Some customers have successfully used this antibody on rat samples, however we have not been successful detecting Myogenin in this species in our own testing and therefore cannot guarantee rat reactivity. Please contact Abcam Scientific Support for more information.</p>

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보완 제품

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Primary Antibodies

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Primary Antibodies

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Myogenin also known as MYOG is a muscle-specific transcription factor that plays an important role in the regulation of muscle differentiation and development. It belongs to the MyoD family of basic helix-loop-helix proteins. Myogenin has a molecular weight of approximately 34 to 39 kDa. It is typically expressed in skeletal muscle tissues aiding in the transition from proliferating myoblasts to differentiated myotubes. Immunohistochemistry (IHC) methods often use myogenin antibodies like the 'myogenin anticuerpo' and 'F5D' to identify myogenin expression within tissue samples facilitating the understanding of muscle tissue organization and development.
Biological function summary

Myogenin functions in controlling the transcription of muscle-specific genes that are essential during myogenesis. This transcription factor forms heterodimers with E-proteins becoming part of DNA-binding complexes that activate the transcription of muscle-specific genes. Myogenin's presence is critical during muscle cell fusion and maturation signifying its importance in proper muscle tissue formation and maintenance. Myogenin staining is often used as a technique to study muscle differentiation in various experimental settings.

Pathways

Myogenin is integrated into the myogenic regulatory network where it activates genes involved in muscle fiber maturation. It participates in the MyoD regulatory pathway working alongside other myogenic regulatory factors like MyoD Myf5 and MRF4. These proteins create a coordinated network ensuring efficient muscle cell differentiation and growth. The cross-talk between myogenin and these factors highlights its role in the intricate web of gene regulation necessary for muscle development.

The altered expression or mutation of the myogenin gene has potential implications in muscular dystrophies and myopathies. For instance disruptions in myogenin activity can lead to impaired muscle repair and regeneration contributing to the pathology of conditions such as rhabdomyosarcoma. Within these contexts myogenin associates with other proteins like MyoD whose expression might also be altered further accentuating the disruption in normal muscle differentiation processes during disease states.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Also cooperates with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Also plays a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.
See full target information Myog

대체 명칭 보기

Myogenin, Myog
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Product promise

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