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AB322729

Alexa Fluor® 647 Anti-Puromycin antibody [EPR27218-173]

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Rabbit Recombinant Monoclonal Puromycin antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Chemical samples. Cited in 1 publication.
2 이미지
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Puromycin antibody [EPR27218-173] (AB322729)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Puromycin antibody [EPR27218-173] (AB322729)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Puromycin with ab322729 at 1/250 (2.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in HeLa cells after treatment with 1 uM Puromycin for 30 mins, while showing no staining in untreated HeLa cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Puromycin antibody [EPR27218-173] (AB322729)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Puromycin antibody [EPR27218-173] (AB322729)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 1uM Puromycin for 30min (Green) / Untreated HeLa (Magenta) cells labelling Puromycin with ab322729 at 1/500 dilution (0.1ug) (Magenta) and (Green) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) and (Grey).

관련 conjugated 항체와 다양한 조성의 항체 (3)

  • Unconjugated

    Anti-Puromycin antibody [EPR27218-173]

  • Carrier free

    Anti-Puromycin antibody [EPR27218-173] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Puromycin antibody [EPR27218-173]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27218-173

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB2254

Anti-Aurora B antibody

BOND RX™ Validated

Western blot - Anti-Aurora B antibody (AB2254)

  • 4
  • 36
Primary Antibodies

AB315887

Anti-Puromycin antibody [EPR27218-173]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Puromycin antibody [EPR27218-173] (AB315887)

  • 5
  • 1
Assay Kits

AB197010

MTS Assay Kit (Cell Proliferation) (Colorimetric)

Biochemical assay - MTS Assay Kit (Cell Proliferation) (Colorimetric) (AB197010)

  • 0
  • 0
Primary Antibodies

AB66217

Anti-ABCA1 antibody [HJ1]

KO Validated|BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] (AB66217)

  • 3
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Puromycin is a well-known antibiotic that acts by disrupting protein synthesis. It mimics the structure of an aminoacyl-tRNA leading to premature termination of the growing polypeptide chain during translation. Puromycin is small with a molecular mass of approximately 471 daltons. It interacts mainly within the cytoplasm where it can incorporate into the peptide chain due to its similarity to the 3' end of the aminoacyl-tRNA. Puromycin does not have a specific expression site like endogenous proteins because it is not naturally occurring in animals; rather it is used experimentally.
Biological function summary

The function of puromycin is to interfere with protein synthesis by targeting ribosomes. It binds to the ribosomal A site leading to the release of incomplete polypeptides. Puromycin is not part of a complex but establishes a strong interaction with ribosomes found in prokaryotic and eukaryotic cells. This interaction gives it unique utility in experiments that require inhibition of translation such as studies involving protein turnover and synthesis rates.

Pathways

The action of puromycin strongly impacts protein synthesis pathways. It inhibits the elongation stage of the translation pathway by mimicking an aminoacyl-tRNA. Puromycin does not involve complex pathways itself but its effects can influence other proteins involved in translation such as elongation factors and the ribosomal subunits. This makes puromycin useful in basic scientific research to study how cells control protein synthesis.

Puromycin's ability to inhibit translation links it to studies on cancer and neurodegenerative diseases. Researchers use puromycin to explore how disrupting protein synthesis can affect cancer cell growth. It serves as a model for understanding toxins that affect similar pathways in neurodegenerative disorders. Through these studies connections have been made between puromycin's action and proteins involved in these diseases such as oncogenes and neuromodulatory proteins which are key to understanding disease progression and developing potential treatments.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information Puromycin
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 16:1725904 PubMed41583458

2026

Hemoglobin alpha regulates T-lymphocyte activation and mitochondrial function.

Applications

Flow Cyt (Intra)

Species

Unspecified reactive species

Emily C Reed,Tatlock H Lauten,Tamara Natour,Lauren J Pitts,Caroline N Jojo,Brooke L Griffin,Sreeram Pasupuleti,Adam J Case
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com