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AB322983

Alexa Fluor® 680 Anti-ICAM1 antibody [EPR22161-284]

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Rabbit Recombinant Monoclonal ICAM1 antibody - conjugated to Alexa Fluor® 680. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human, Mouse samples.
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Flow Cytometry - Alexa Fluor® 680 Anti-ICAM1 antibody [EPR22161-284] (AB322983)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 680 Anti-ICAM1 antibody [EPR22161-284] (AB322983)

Flow cytometry overlay histogram showing left A-20 positive cells and right negative L-929 stained with ab322983 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein followed by the antibody (ab322983) (1x 106 in 100 ul at 0.04 ug/ml (1/12500)) for 30min on ice.

Isotype control antibody (black line) was Alexa Fluor® 680 Rabbit IgG, monoclonal Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 712/25 bandpass filter.

관련 conjugated 항체와 다양한 조성의 항체 (5)

  • Unconjugated

    Anti-ICAM1 antibody [EPR22161-284]

  • Carrier free

    Anti-ICAM1 antibody [EPR22161-284] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ICAM1 antibody [EPR22161-284]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284]

  • 519 FITC

    FITC Anti-ICAM1 antibody [EPR22161-284]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22161-284

Isotype

IgG

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Carrier free

No

Reacts with

Human, Human, Mouse

Applications

Flow Cyt, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB174445

Human ICAM1 ELISA Kit (CD54)

SimpleStep

ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

  • 0
  • 0
Primary Antibodies

AB194319

Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (AB194319)

  • 5
  • 1
Primary Antibodies

AB317067

Alexa Fluor® 555 Anti-VE Cadherin antibody [EPR27436-55]

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-VE Cadherin antibody [EPR27436-55] (AB317067)

  • 0
  • 0
Primary Antibodies

AB318169

Alexa Fluor® 488 Anti-CD62E antibody [EPR25616-62]

Recombinant|RabMAb

Flow Cytometry - Alexa Fluor® 488 Anti-CD62E antibody [EPR25616-62] (AB318169)

  • 0
  • 0
Primary Antibodies

AB281580

Alexa Fluor® 647 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (AB281580)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/12500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM : ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1.
See full target information Icam1

대체 명칭 보기

CD54, Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10, Icam1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com