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AB269290

Alexa Fluor® 700 Anti-CD62L antibody [DREG-56]

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Anti-CD62L antibody [DREG-56] conjugated to Alexa Fluor® 700 - suitable for flow cytometry, and reacts with human samples. Clone [DREG-56] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - Alexa Fluor® 700 Anti-CD62L antibody [DREG-56] (AB269290)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 700 Anti-CD62L antibody [DREG-56] (AB269290)

Flow cytometry analysis (surface staining) of CD62L in human peripheral blood labeling CD62L with ab269290 (4 µl reagent / 100 µl of peripheral whole blood).

Flow Cytometry - Alexa Fluor® 700 Anti-CD62L antibody [DREG-56] (AB269290)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 700 Anti-CD62L antibody [DREG-56] (AB269290)

Separation of human neutrophil granulocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab269290 (4 µl reagent / 100 µl of peripheral whole blood).

관련 conjugated 항체와 다양한 조성의 항체 (4)

  • 660 APC

    APC Anti-CD62L antibody [DREG-56]

  • 519 FITC

    FITC Anti-CD62L antibody [DREG-56]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD62L antibody [DREG-56]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD62L antibody [DREG-56]

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

DREG-56

Isotype

IgG1

Conjugation

Alexa Fluor® 700

Excitation/Emission

Ex: 702nm, Em: 723nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing SELL protein. The exact immunogen used to generate this antibody is proprietary information.

P14151

Specificity

ab269290 recognizes an extracellular epitope of CD62L.

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대체품

Primary Antibodies

AB253240

Anti-CD62L antibody [EPR17012-27]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (AB253240)

  • 0
  • 0
Primary Antibodies

AB253241

Anti-CD62L antibody [EPR17012-35]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-35] (AB253241)

  • 0
  • 0
Primary Antibodies

AB254203

Anti-CD62L antibody [EPR17012-27] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (AB254203)

  • 0
  • 0
Primary Antibodies

AB254204

Anti-CD62L antibody [EPR17012-35] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD62L antibody [EPR17012-35] - BSA and Azide free (AB254204)

  • 0
  • 0

보완 제품

Assay Kits

AB45917

Human CD62L ELISA Kit (Selectin L)

Sandwich ELISA - Human CD62L ELISA Kit (Selectin L) (AB45917)

  • 0
  • 0
Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Or 100 μL of whole blood.</p>" } } }
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제품 세부 정보

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Size-exclusion chromatography
Purification 관련 사항
The purified antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
보관 버퍼
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed : 12403782, PubMed : 28011641, PubMed : 28489325). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed : 12403782, PubMed : 28011641).
See full target information SELL

대체 명칭 보기

CD62L, LNHR, LYAM1, SELL, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, TQ1, gp90-MEL, LAM-1, LECAM1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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