AB184577

Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control

Name: Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control
Brand: Abcam Limited
SKU: AB184577
Rating: 5 (2 reviews)

(2 리뷰들)

(4 제품이 사용된 논문 )

Anti-alpha Tubulin antibody [DM1A] conjugated to Alexa Fluor® 790 - suitable for Western blot, and reacts with mouse, human samples. Clone [DM1A] is a mouse monoclonal antibody cited in 28 publications.

1 이미지

Lab

Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB184577)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184577 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

DM1A

Isotype

IgG1

Light chain 타입

kappa

Conjugation

Alexa Fluor® 790

Excitation/Emission

Ex: 782nm, Em: 805nm

Carrier free

Reacts with

Mouse, Human

Applications

applications

Epitope

aa 426-450

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

대체 명칭 보기

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2426909122 PubMed40504161

2025

Defining CDK12 as a tumor suppressor and therapeutic target in mouse models of tubo-ovarian high-grade serous carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Ching-Yi Tien,Yali Zhai,Rong Wu,Yuping Zhang,Yu Chang,Yunhui Cheng,Abigail J Todd,Christina E Wheeler,Shuqin Li,Rahul Mannan,Caleb Cheng,Brian Magnuson,Gabriel Cruz,Yizhi Cao,Somnath Mahapatra,Carmine Stolfi,Xuhong Cao,Fengyun Su,Rui Wang,Jianzhang Yang,Licheng Zhou,Yuanyuan Qiao,Lanbo Xiao,Marcin Cieslik,Xiaoju Wang,Zhen Wang,Jonathan Chou,Eric R Fearon,Ke Ding,Kathleen R Cho,Arul M Chinnaiyan

Cell reports. Medicine 5:101752 PubMed39353441

2024

Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chang,Xiaoju Wang,Jianzhang Yang,Jean Ching-Yi Tien,Rahul Mannan,Gabriel Cruz,Yuping Zhang,Josh N Vo,Brian Magnuson,Somnath Mahapatra,Hanbyul Cho,Saravana Mohan Dhanasekaran,Cynthia Wang,Zhen Wang,Licheng Zhou,Kaijie Zhou,Yang Zhou,Pujuan Zhang,Weixue Huang,Lanbo Xiao,Weihuang Raymond Liu,Rudana Hamadeh,Fengyun Su,Rui Wang,Stephanie J Miner,Xuhong Cao,Yunhui Cheng,Rohit Mehra,Ke Ding,Arul M Chinnaiyan

Nature communications 13:3750 PubMed35768438

2022

The genetic heterogeneity and drug resistance mechanisms of relapsed refractory multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Josh N Vo,Yi-Mi Wu,Jeanmarie Mishler,Sarah Hall,Rahul Mannan,Lisha Wang,Yu Ning,Jin Zhou,Alexander C Hopkins,James C Estill,Wallace K B Chan,Jennifer Yesil,Xuhong Cao,Arvind Rao,Alexander Tsodikov,Moshe Talpaz,Craig E Cole,Jing C Ye,P Leif Bergsagel,Daniel Auclair,Hearn Jay Cho,Dan R Robinson,Arul M Chinnaiyan

American journal of cancer research 9:585-596 PubMed30949412

2019

Decreased miR-124-3p promoted breast cancer proliferation and metastasis by targeting MGAT5.

Applications

Unspecified application

Species

Unspecified reactive species

Guiling Yan,Yinhui Li,Lu Zhan,Shuhan Sun,Jihang Yuan,Tiantian Wang,Yupeng Yin,Zhihui Dai,Yiqing Zhu,Zhijing Jiang,Lin Liu,Yinxing Fan,Fu Yang,Wei Hu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control

Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB184577)

관련 conjugated 항체와 다양한 조성의 항체 (7)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Epitope

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise