AB81652

Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10]

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Anti-6X His tag® antibody [AD1.1.10] conjugated to Alkaline Phosphatase (Alk Phos) - suitable for Western blot. Clone [AD1.1.10] is a mouse monoclonal antibody cited in 79 publications.

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Supplier Data

Western blot - Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (AB81652)

Imaged using stabilized Alkaline Phosphatase substrate.

All lanes:

Western blot - Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652) at 1/10000 dilution

Lane 1:

60 kDa 6X His-tagged protein at 0.3 µg

Lane 2:

60 kDa 6X His-tagged protein at 0.1 µg

Lane 3:

60 kDa 6X His-tagged protein at 0.03 µg

Lane 4:

60 kDa 6X His-tagged protein at 0.01 µg

Lane 5:

60 kDa 6X His-tagged protein at 0.003 µg

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

AD1.1.10

Isotype

IgG1

Conjugation

Alkaline Phosphatase

Excitation/Emission

Carrier free

Applications

applications

Specificity

ab81652 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

보관 버퍼

pH: 7.2 Preservative: 0.013% Sodium azide Constituents: 0.87% Sodium chloride, 0.3025% Tris

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information 6X His tag®

대체 명칭 보기

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

mBio 11: PubMed33144373

2020

EPEC Recruits a Cdc42-Specific GEF, Frabin, To Facilitate PAK Activation and Host Cell Colonization.

Applications

Unspecified application

Species

Unspecified reactive species

Vikash Singh,Peter J Hume,Anthony Davidson,Vassilis Koronakis

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10]

Western blot - Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (AB81652)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise