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AB6661

Alkaline Phosphatase Anti-GFP antibody

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(3 제품이 사용된 논문 )

Anti-GFP antibody conjugated to Alkaline Phosphatase (Alk Phos) is a goat polyclonal antibody suitable for Dot, ELISA, Western blot.
2 이미지
Western blot - Alkaline Phosphatase Anti-GFP antibody (AB6661)
  • WB

Supplier Data

Western blot - Alkaline Phosphatase Anti-GFP antibody (AB6661)

All lanes:

Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661) at 1/1000 dilution

All lanes:

GFP at 0.05 µg

Predicted band size: 27 kDa

false

Dot Blot - Alkaline Phosphatase Anti-GFP antibody (AB6661)
  • Dot

Supplier Data

Dot Blot - Alkaline Phosphatase Anti-GFP antibody (AB6661)

Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.

Lane 1 : 100 ng GFP protein
Lane 2 : 33.33 ng GFP protein
Lane 3 : 11.11 ng GFP protein
Lane 4 : 3.70 ng GFP protein
Lane 5 : 1.23 ng GFP protein

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Alkaline Phosphatase

Excitation/Emission
Carrier free

No

Applications

ELISA, WB, Dot

applications

Immunogen

Recombinant Full Length Protein corresponding to Aequorea victoria GFP.

P42212

Specificity

<p>Antibody recognizes wild type, recombinant and enhanced forms of GFP. Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. No reaction was observed against human, mouse and rat serum proteins.</p>

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>This product has been assayed against 1.0 μg of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.</p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

제품 세부 정보

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.

Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
보관 버퍼
pH: 8 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP

대체 명칭 보기

Green fluorescent protein, GFP

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Applied microbiology and biotechnology 106:1677-1689 PubMed35129657

2022

Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley E Sproles,Anthony Berndt,Francis J Fields,Stephen P Mayfield

PloS one 16:e0257089 PubMed34793485

2021

Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony J Berndt,Tressa N Smalley,Bijie Ren,Ryan Simkovsky,Amr Badary,Ashley E Sproles,Francis J Fields,Yasin Torres-Tiji,Vanessa Heredia,Stephen P Mayfield

PloS one 8:e59435 PubMed23555033

2013

Fission yeast CSL proteins function as transcription factors.

Applications

WB

Species

Unspecified reactive species

Martina Oravcová,Mikoláš Teska,František Půta,Petr Folk,Martin Převorovský
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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