AB195379

Anti-ALKBH4 antibody [EPR19216]

Name: Anti-ALKBH4 antibody [EPR19216]
Brand: Abcam Limited
SKU: AB195379
Rating: 5 (1 reviews)

RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal ALKBH4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

ABH4, ALKBH4, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, Alkylated DNA repair protein alkB homolog 4, DNA N6-methyl adenine demethylase ALKBH4, Lysine-specific demethylase ALKBH4

3 이미지

Supplier Data

Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (AB195379)

ALKBH4 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab195379 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab195379 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HEK-293 whole cell lysate 10ug (Input).
Lane 2 : ab195379 IP in HEK-293 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab195379 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (ab195379)

Predicted band size: 34 kDa

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Supplier Data

Western blot - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Blocking/Dilution : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 33 kDa

true

Exposure time: 1min

Supplier Data

Western blot - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Blocking/Dilution : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 4:

Human pancreas tissue lysate at 10 µg

Lane 5:

WI-38 (human fetal lung fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 33 kDa

true

Exposure time: 1min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19216

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Primates": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes (PubMed : 23673617). Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed : 23673617). In addition to proteins, also demethylates DNA : specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity).

See full target information ALKBH4

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 28:113520 PubMed41069850

2025

ALKBH5-IGF2BP2 axis mediates prostate cancer progression and docetaxel resistance via m6A-stabilized CLSPN RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Chen,Yang Li,Zongzhu Liang,Zhao Yang,Haotian Ren,Wanqing Wei,Yuanjie Niu,Zhiqun Shang

Scientific reports 11:8677 PubMed33883577

2021

ALKBH4 promotes tumourigenesis with a poor prognosis in non-small-cell lung cancer.

Applications

Species

Human

Kentaro Jingushi,Masaya Aoki,Kazuhiro Ueda,Takahiro Kogaki,Masaya Tanimoto,Yuya Monoe,Masayuki Ando,Takuya Matsumoto,Kentaro Minami,Yuko Ueda,Kaori Kitae,Hiroaki Hase,Toshiyuki Nagata,Aya Harada-Takeda,Masatatsu Yamamoto,Kohichi Kawahara,Kazuhiro Tabata,Tatsuhiko Furukawa,Masami Sato,Kazutake Tsujikawa

EBioMedicine 59:102955 PubMed32853985

2020

N6-Methyladenosine modification of the TRIM7 positively regulates tumorigenesis and chemoresistance in osteosarcoma through ubiquitination of BRMS1.

Applications

Unspecified application

Species

Unspecified reactive species

Chenliang Zhou,Zhichang Zhang,Xiaoshi Zhu,Guowei Qian,Yan Zhou,Yong Sun,Wenxi Yu,Jiahui Wang,Haiyang Lu,Feng Lin,Zan Shen,Shuier Zheng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALKBH4 antibody [EPR19216]

Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Western blot - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Western blot - Anti-ALKBH4 antibody [EPR19216] (AB195379)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise