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AB230695

Anti-alpha 1 Antitrypsin antibody [11G2]

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Mouse Monoclonal alpha 1 Antitrypsin antibody. Suitable for IHC-P, IP, WB and reacts with Human, Dog, African green monkey, Transfected cell lysate - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINA1.

대체 명칭 보기

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

Formalin-fixed, paraffin-embedded human liver tissue stained for alpha 1 Antitrypsin using ab230695 at 10 μg/ml in immunohistochemical analysis.

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)
  • IP

Supplier Data

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

alpha 1 Antitrypsin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) DDK tagged alpha 1 Antitrypsin antibody overexpression lysate with 2 μg ab230695 and 20 μl (0.1 mg) of goat anti-mouse conjugated magnetic beads. Western blot was performed from the immunoprecipitate using rabbit anti-DDK polyclonal antibody.

Lane 1 : Negative control : IP without adding anti-alpha 1 Antitrypsin antibody in HEK-293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.

Lane 2 : ab230695 IP in -293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.

All lanes:

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)

Predicted band size: 46 kDa

false

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)
  • WB

Supplier Data

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 46 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

11G2

Isotype

IgG1

Carrier free

No

Reacts with

Dog, Human, African green monkey

Applications

IP, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SERPINA1.

P01009

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대체품

Primary Antibodies

AB166610

Anti-alpha 1 Antitrypsin antibody [EPR9090]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR9090] (AB166610)

  • 3
  • 1
Primary Antibodies

AB133642

Anti-alpha 1 Antitrypsin antibody [EPSISR16]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (AB133642)

  • 0
  • 0
Primary Antibodies

AB207303

Anti-alpha 1 Antitrypsin antibody [EPR17087-50]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (AB207303)

  • 4
  • 2
Primary Antibodies

AB167414

Anti-alpha 1 Antitrypsin antibody [EPR10832(B)]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPR10832(B)] (AB167414)

  • 0
  • 0
Primary Antibodies

AB206735

Alexa Fluor® 647 Anti-alpha 1 Antitrypsin antibody [EPR9090]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha 1 Antitrypsin antibody [EPR9090] (AB206735)

  • 0
  • 0

보완 제품

Assay Kits

AB189579

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)

SimpleStep

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)

  • 0
  • 0
Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

  • 5
  • 2
Primary Antibodies

AB169552

Anti-alpha 1 Fetoprotein antibody [EPR9309]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPR9309] (AB169552)

  • 0
  • 0
Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

  • 5
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/µL", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from TCS by Protein A/G.
보관 버퍼
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.
Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
See full target information SERPINA1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 9:eade9459 PubMed36662852

2023

Inflammation of the retinal pigment epithelium drives early-onset photoreceptor degeneration in -associated retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Maria E Mercau,Yemsratch T Akalu,Francesca Mazzoni,Gavin Gyimesi,Emily J Alberto,Yong Kong,Brian P Hafler,Silvia C Finnemann,Carla V Rothlin,Sourav Ghosh
제품이 사용된 논문 모두 보기
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Product promise

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